1. Identification and analysis of a SMAD3 cis-acting eQTL operating in primary osteoarthritis and in the aneurysms and osteoarthritis syndrome 
E.V.A. Raine, L.N. Reynard, I.M.B.H. van de Laar, A.M. Bertoli-Avella, J. Loughlin 
Osteoarthritis and Cartilage 
Volume 22, Issue 5, Pages (May 2014)
DOI: /j.joca
Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

2. Fig. 1
Columnar scatter plots of the quantitative expression of SMAD3 in knee OA vs hip OA cartilage. n is the number of patients studied. The horizontal lines in each plot represent the mean and the 95% confidence interval. P-value was calculated using a Mann–Whitney U Test.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

3. Fig. 2
Allelic expression analysis of the 3'UTR transcript SNP rs using cDNA from cartilage. The genotypes of the patients for the associated SNP rs are shown. The cDNA allelic ratio in each patient was compared to the 1:1 allelic ratio calculated from the DNA of that patient.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

4. Fig. 3
Columnar scatter plots of the quantitative expression data of SMAD3. (A) shows OA cartilage, (B) shows OA knee cartilage, and (C) shows OA hip cartilage, with patients stratified by genotype at the 3′UTR transcript SNP rs Due to the low frequency of AA homozygotes at rs the analysis was performed between GG homozygotes and A-allele carriers (GA and AA combined). n is the number of patients studied. The horizontal lines in each plot represent the mean and the 95% confidence interval. P-values were calculated using a Mann–Whitney U Test.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

5. Fig. 4
Functional analysis of the six SMAD3 3'UTR SNPs in SW1353 cells. (A–C) show the results of the luciferase reporter assays for amplicons A, B and C, respectively. (D) shows the results of the luciferase reporter assay for the four possible haplotypes (CC, CT, TT, and TC) for rs and rs Data are the fold expression relative to Renilla activity and are shown as the mean and 95% confidence intervals from three biological repeats each with six technical replicates. P-values were calculated using a Mann–Whitney U Test.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

6. Fig. 5
Functional analysis of the six SMAD3 3'UTR SNPs in MG63 cells. (A–C) show the results of the luciferase reporter assays for amplicons A, B, and C, respectively. (D) shows the results of the luciferase reporter assay for the four possible haplotypes (CC, CT, TT, and TC) for rs and rs Data are the fold expression relative to Renilla activity and are shown as the mean and 95% confidence intervals from three biological repeats each with six technical replicates. P-values were calculated using a Mann–Whitney U Test.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

7. Fig. 6
Allelic expression analysis of the 3'UTR SNP rs using cDNA from vascular smooth muscle cells. The cDNA allelic ratio in each individual was compared to the 1:1 allelic ratio calculated from the DNA of that individual.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions