1. Immunoblot analysis of apoptotic HCT-8 cell extracts separated by SDS-PAGE.
Immunoblot analysis of apoptotic HCT-8 cell extracts separated by SDS-PAGE. Proteins were electroblotted onto nitrocellulose membranes and incubated with specific IgG (lanes 1–4, monoclonal IgG against stathmin; lanes 5–8, polyclonal IgG against glucosidase II β subunit). IgGs were revealed with their specific peroxidase-conjugated goat IgG. The immunoblots were developed using the ECL detection system. Negative controls were performed without incubating membranes with anti-stathmin or anti-glucosidase II β subunit antibodies. Lane 1, stathmin, 2 mm Gln; lane 2, stathmin, 10 mm Gln; lane 3, negative control, 2 mm Gln; lane 4, negative control, 10 mm Gln; lane 5, glucosidase II β subunit, 2 mm Gln; lane 6, glucosidase II β subunit, 10 mm Gln; lane 7, negative control, 2 mm Gln; lane 8, negative control, 10 mm Gln.
Nicolas Deniel et al. Mol Cell Proteomics 2007;6:
© 2007 The American Society for Biochemistry and Molecular Biology