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Enhanced Expression of Human Metalloelastase (MMP-12) in Cutaneous Granulomas and Macrophage Migration  Maarit Vaalamo, Arja-Leena Kariniemi, Ulpu Saarialho-Kere 

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Presentation on theme: "Enhanced Expression of Human Metalloelastase (MMP-12) in Cutaneous Granulomas and Macrophage Migration  Maarit Vaalamo, Arja-Leena Kariniemi, Ulpu Saarialho-Kere "— Presentation transcript:

1 Enhanced Expression of Human Metalloelastase (MMP-12) in Cutaneous Granulomas and Macrophage Migration  Maarit Vaalamo, Arja-Leena Kariniemi, Ulpu Saarialho-Kere  Journal of Investigative Dermatology  Volume 112, Issue 4, Pages (April 1999) DOI: /j x Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 HME is expressed in sarcoid granulomas in the same areas as MMP-9. (a) Expression of HME in histiocytes of sarcoid granulomas. (b) Serial section stained for elastin. (c) Bright-field photo of cells expressing HME mRNA. (d) Staining for HME protein in a serial section. Arrows mark corresponding areas. (inset d′) Double-staining reveals that a subpopulation of CD68 positive macrophages (red) express HME protein (dark blue) in the same specimen. Arrow depicts a double-stained cell. (e) Bright-field photo of HME mRNA in a sarcoid granuloma. (f) Serial section immunostained for uPA. (g) Signal for HME mRNA in another sample of sarcoidosis. (h) Immunostaining for MMP-9 reveals this protein in the same areas. (i) Positive immunostaining for MMP-2 around the granuloma. Arrows show the location of MMP-2 positive cells. (j) Higher magnification showing MMP-2 in fibroblast-like cells. Counterstaining with hematoxylin and eosin (a, c, e, g) or with hematoxylin (d, inset d′, f, h, i, j). Scale bars: (a, b, g–i) 40 μm; (c–f) 20 μm; (j) 10 μm. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 HME is expressed by macrophages in NLD. (a) In situ hybridization with anti-sense HME probe in a sample of NLD. (b) A negative control hybridized with HME sense probe. (c) A serial section with staining for elastic fibres. (d) In situ hybridization with HME anti-sense probe. (e) Immunostaining for CD68. (inset f′) Double-staining reveals that a subpopulation of CD68 positive macrophages (red) express HME protein (dark blue). Arrows depict double-stained cells. (f) Dark-field photomicrograph with signal for HME mRNA. (g) Corresponding section stained for HME protein. Arrows depict corresponding areas. Counterstaining with hematoxylin and eosin (a, b, d, f) or with hematoxylin (e, inset f′, g). Scale bars: (a–c, f, g) 40 μm; (d, e) 20 μm; (inset f′) 10 μm. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 HME is expressed in macrophages in areas devoid of elastin in granuloma annulare. (a) Expression of HME in histiocytes. (b) Staining for elastic fibres. (c) Dark-field of another sample of granuloma annulare hybridized with HME anti-sense probe. (inset c′) Double-staining reveals that a subpopulation of CD68 positive macrophages (red) express HME protein (dark blue) in the same specimen. Arrows depict double-stained cells. (d) Immunostaining for HME protein in an adjacent section. (e) Higher magnification of cells positive for HME protein. (f) Expression of HME in a necrobiotic area. (g) A nearby section immunostained for MMP-9. (h) Immunostaining for uPA. (i) Dark-field view of HME mRNA expressing cells in a nearby section. Counterstaining with hematoxylin and eosin (a, c, f, i) or with hematoxylin (inset c′, d, e, g, h). Scale bars: (a–d, h, i) 40 μm; (e–g) 20 μm; (inset c′) 10 μm. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 HME is expressed by migrating macrophages associated with areas of BM disruption in pityriasis lichenoides and DH. (a) In situ hybridization with anti-sense HME probe in a sample of pityriasis lichenoides. (b) Immunostainings for CD68 and for MMP-9 (c) in the same specimen. (d) Another sample on pityriasis lichenoides with signal for HME. (e) Staining for type IV collagen in a serial section. Long arrow depicts the disappearance of type IV collagen staining, small arrows corresponding areas. (f) In situ hybridization dark-field view for HME anti-sense probe in a sample of DH. Counterstaining with hematoxylin and eosin (a, d, f) or with hematoxylin (b, c, e). Scale bars: (a, d–f) 40 μm; (b, c) 20 μm. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

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