1. 4/16 Daily Catalyst Pg. 53 DNA Extraction Lab
1. Label the parts of DNA:
2. Provide an example of a deletion mutation.
3. In gel electrophoresis, DNA separates based on _________.
4. What gives DNA is charge? A B C

2. 4/16 Daily Catalyst Pg. 53 DNA Extraction Lab
Quiz #12 DNA on Friday
DNA test 4/22
Study guide due test
Review day on 4/21
DNA Extraction lab on Thursday
Be here! Labs are test grades!
Interims come out this Friday
Signed for extra credit
Parent Night on Thursday 6-7:30
Extra Credit

3. 4/16 Agenda Pg. 53 DNA Extraction Lab
Daily Catalyst
Class Business
Objective
DNA extraction lab
Clean up
Homework: lab report

4. DNA Recap 1. What are the three parts to DNA?
Sugar, phosphate, and base
2. In base pairing, A pairs with _______ and C pairs with ____.
A-T
C-G
3. Who discovered DNA?
Watson, Crick, and Franklin
4. What is the structure of DNA?
Double helix

5. 4/16 Objective
Today, we will demonstrate the presence of DNA in eukaryotic cells using a DNA extraction technique.

6. Key Point #1: Extraction:
To remove

7. Key Point #2: Living Cells
Prokaryotes
Eukaryotes
Bacteria
Contain DNA
Double or single helix
Animals and Plants
Contain DNA
Double helix

8. DNA Extraction Key Point #3: Key Point #4:
DNA is extracted from the nucleus
Key Point #4:
The nuclear membrane must be broken to release the DNA
Break the phospholipid membrane

9. Rules for the lab
We will stay with our assigned group at our assigned lab table.
We will read ALL directions before beginning the lab
All materials will be used safely and properly
Materials will be returned to their proper place and CLEANED
Our lab space will be spotless at the end of the lab
Failure to violate one of the rules will be immediate removal from the classroom and a score of a zero on the lab

10. Lab Groups Lab 1 Rilda & Kaitlyn Romel & Malika Lab 2 Diana & Victoria
Strawberry
Banana
Lab 1
Rilda & Kaitlyn
Romel & Malika
Lab 2
Diana & Victoria
Kyla & Mario
Lab 3
Christian & Hannah O.
Odalys & Mohammad
Lab 4
Lawren & Mr. Kaki
Irma & Julianna
Lab 5
Sierra & Tiesha
Michelle & Camila
Esther & Lavonne
Hannah L., James & Joseph

11. DNA extraction lab
Today, we will extract DNA from fruit. Each group will have a fruit piece then we will compare results at the end. For this lab to be successful, all directions need to be carefully read.

12. DNA extraction lab
Directions: With your partner, complete the pre-reading and pre-lab questions. Assign the jobs to your partnership and create the perfect hypothesis for an amazing lab report.
Time: 10 minutes
Noise: 1 (whispers)

13. Filter folding