1. Leukotriene D4 enhances immunoglobulin production in CD40-activated human B lymphocytes 
Josée Lamoureux, PhD, Jana Stankova, PhD, Marek Rola-Pleszczynski, MD 
Journal of Allergy and Clinical Immunology 
Volume 117, Issue 4, Pages (April 2006)
DOI: /j.jaci
Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

2. Fig 1
A, Modulation of expression of CysLT1 in CD19+ cells from PBLs by anti-CD40 Ab (G28.5; 5 μg/mL), IL-4 (10 ng/mL), PMA (10−7 mol/L), TNF-α (20 ng/mL), IFN-γ (100 U/mL) or PAF (10−7 mol/L). PBLs were stimulated for 48 (gray bars) and 72 (black bars) hours before analysis by flow cytometry. B, Expression of CysLT1 in purified CD19+ B lymphocytes. Cells were stimulated by coculture with irradiated murine L929 fibroblasts or with CD154-transfected L929 fibroblasts (L4.5) in the absence or presence of IL-4 (100 U/mL) or IL-13 (20 ng/mL) for 48 (gray bars) and 72 (black bars) hours before analysis by flow cytometry. Geometrical mean fluorescence intensity ± SEM is presented; n = 3-7; ∗P < .05.
Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )
Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

3. Fig 2
Modulation of expression of CysLT1 in purified B lymphocytes stimulated in the CD154 system. Cells were stimulated through a 72-hour time course and harvested at indicated times. Steady state levels of CysLT1 and glyceraldehyde phosphate dehydrogenase mRNA were measured by semiquantitative RT-PCR (A) and further quantified by densitometry (B). Control lane was PCR reaction in absence of RT. n = 3.
Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )
Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

4. Fig 3
A, Calcium mobilization in CD40-activated B lymphocytes. Cells were stimulated for 72 hours in presence or absence of IL-4 in combination with untransfected (L929) or CD154-transfected (L4.5) fibroblasts. Cells were then loaded with Fluo-3 before stimulation with 10−7 mol/L LTD4 and analysis by flow cytometry. B, Cells were stimulated with 10−7 mol/L LTD4 in the absence (plain line) or presence of pretreatment with 10−7 mol/L montelukast (dotted line). Arrows indicate the time of leukotriene addition. These graphics are representative of 3 to 5 separate experiments.
Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )
Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

5. Fig 4
Expression of mature epsilon transcripts in CD40-activated B lymphocytes exposed to graded concentrations of LTD4. Cells were stimulated as described for 14 days, and RNA expression of mature epsilon transcript and GAPDH control gene was measured in semiquantitative RT-PCR. Densitometry was used to calculate the epsilon/GAPDH ratio, which was normalized to 100% for control, LTD4-unstimulated cultures. Each treatment was then compared with the LTD4-unstimulated condition. Data are means ± SEMs; n = 2-7; ∗P < .05.
Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )
Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

6. Fig 5
Production of immunoglobulins by IL-4 and CD40-activated B lymphocytes after treatment with graded concentrations of LTD4. Upper panels, Secretion of IgG (A), IgM (B), and IgE (C) was measured by ELISA in supernatants of B cells after 14 days of costimulation with IL-4, CD154-transfected fibroblasts, and graded concentrations of LTD4. Lower panels, Producers of IgG (D), IgM (E), and IgE (F) were quantified by ELISPOT using B cells after 14 days of costimulation with IL-4, CD154-transfected fibroblasts, and graded concentrations of LTD4. The production level of each treatment was then compared with the LTD4-unstimulated condition, normalized to 100%. Data are means ± SEMs; n = 3-10; ∗P < .05.
Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )
Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions