1. Messenger RNA expression for the follicle-stimulating hormone receptor and luteinizing hormone receptor in human oocytes and preimplantation-stage embryos 
Eleni Patsoula, PhD, Dimitris Loutradis, MD, Peter Drakakis, MD, Lina Michalas, MD, Ritsa Bletsa, RN, Stylianos Michalas, MD 
Fertility and Sterility 
Volume 79, Issue 5, Pages (May 2003)
DOI: /S (03)

2. FIGURE 1
FSHR mRNA expression in human oocytes and preimplantation embryos. M: 100 bp DNA marker; lane 1: unfertilized oocytes with and without RT, respectively (±); lane 2: zygotes (±); lane 3: 2-cell embryos (±); lane 4: 4-cell embryos (±); lane 5: morula-stage embryos (±); lane 6: blastocyst-stage embryos (±).
Patsoula. FSH, LH receptors in human pre-embryos. Fertil Steril 2003.
Fertility and Sterility  , DOI: ( /S (03) )

3. FIGURE 2
RT-PCR amplification of LHR mRNA in human oocytes and preimplantation embryos. M: 100 bp DNA marker; lane 1: unfertilized oocytes with and without RT, respectively (±); lane 2: zygotes (±); lane 3: 2-cell embryos (±); lane 4: 4-cell embryos (±); lane 5: morula-stage embryos (±); lane 6: blastocyst-stage embryos (±).
Patsoula. FSH, LH receptors in human pre-embryos. Fertil Steril 2003.
Fertility and Sterility  , DOI: ( /S (03) )

4. FIGURE 3
(A) Gel electrophoresis of RT-PCR products for FSHR with mRNA from cumulus cells and with mRNA from culture medium. FSHR RT-PCR analysis with mRNA from cumulus cells surrounding oocytes (lane 1) and with mRNA from cumulus cells surrounding zygotes (lane 2). RT-PCR analysis with culture medium of 2-cell embryos (lane 3), 4-cell embryos (lane 4), morulae (lane 5), and blastocysts (lane 6). (B) Gel electrophoresis of RT-PCR products for LHR with mRNA from cumulus cells and with mRNA from culture medium. LHR RT-PCR analysis with mRNA from cumulus cells surrounding oocytes (lane 7) and with mRNA from cumulus cells surrounding zygotes (lane 8). RT-PCR analysis with culture medium of 2-cell embryos (lane 9), 4-cell embryos (lane 10), morulae (lane 11), and blastocysts (lane 12). A 100-bp molecular size marker was used.
Patsoula. FSH, LH receptors in human pre-embryos. Fertil Steril 2003.
Fertility and Sterility  , DOI: ( /S (03) )

5. FIGURE 4
Restriction enzyme digest of the second-round 340-bp FSHR PCR product (A) and the 191-bp LHR PCR product (B) with the Hind III enzyme. Restriction products are indicated with arrows. A 100-bp molecular size marker was used.
Patsoula. FSH, LH receptors in human pre-embryos. Fertil Steril 2003.
Fertility and Sterility  , DOI: ( /S (03) )