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Evaluation of High-Resolution Melting Analysis as a Diagnostic Tool to Detect the BRAF V600E Mutation in Colorectal Tumors  Martin Pichler, Marija Balic,

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Presentation on theme: "Evaluation of High-Resolution Melting Analysis as a Diagnostic Tool to Detect the BRAF V600E Mutation in Colorectal Tumors  Martin Pichler, Marija Balic,"— Presentation transcript:

1 Evaluation of High-Resolution Melting Analysis as a Diagnostic Tool to Detect the BRAF V600E Mutation in Colorectal Tumors  Martin Pichler, Marija Balic, Elke Stadelmeyer, Christoph Ausch, Martina Wild, Christian Guelly, Thomas Bauernhofer, Hellmut Samonigg, Gerald Hoefler, Nadia Dandachi  The Journal of Molecular Diagnostics  Volume 11, Issue 2, Pages (March 2009) DOI: /jmoldx Copyright © 2009 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Comparison of 238 bp (A and C) and 147 bp (B and D) amplicons containing the BRAF V600E mutation. Melting curves are shown in A and B difference plots are shown in C and D. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2009 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 Comparison of the sensitivities of DHPLC (A), DNA sequencing (B), and HRM assay (C) for the detection of the BRAF V600E mutation. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2009 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

4 Figure 3 Influence of formalin-fixation and paraffin-embedding on HRM analysis. Green melting curves represent different dilutions of mutant DNA in wild-type cell line DNA. All samples and dilutions are displayed in duplicates. In (A) melting curves of 10 wild-type FFPE samples (blue) and one BRAF V600E mutant FFPE sample (red) are depicted. B: Comparison of four corresponding BRAF wild-type cryopreserved (blue) and FFPE tissues (red). C: Preamplification step for FFPE derived DNA with the proofreading polymerase Optimase versus preamplifcation of FFPE samples by conventionally used Taq polymerase. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2009 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

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