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A study of one soil, its relatives and contaminants by arbitrary primed PCR with 50mer based analysis Leigh Burgoyne, Lin Koh, David Catcheside Forensic Science International: Genetics Supplement Series Volume 5, Pages e503-e505 (December 2015) DOI: /j.fsigss Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions
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Fig. 1 Scattergram comparison of the frequency of discriminating 50mer sequences from related and unrelated soil metagenomes. Each data point is a plot of the frequency of occurrence of a specific 50 base oligomer amongst the PCR amplification products of two soil metagenomes; the x-coordinate is the frequency in one sample and the y-coordinate the frequency in the other. (A) Duplicate samples from site MBCR, an urban roadside “nature strip”, taken on the same day. Most data points fall close to a diagonal line, indicating that the 50mer sequences are common to both samples. (B) Samples from soils of different type from widely separated locations. None of the 50mers are shared and all data points fall on the axes. Forensic Science International: Genetics Supplement Series 2015 5, e503-e505DOI: ( /j.fsigss ) Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions
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Fig. 2 This example application shows that the relatedness of soil metagenomes is reduced both by six months separation in time and by one metre separation in space. (A) Relative frequency of 50mers amplified from soil samples taken six months apart from the same location (SDN). (B) Relative frequency of 50mers amplified from soil SDN and a soil sample taken from one metre away. A similar degree of relatedness was observed whenever soils were sampled from locations one metre apart (data not shown). Forensic Science International: Genetics Supplement Series 2015 5, e503-e505DOI: ( /j.fsigss ) Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions
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