1. Surgical Preparation Abolishes Endothelium-Derived Hyperpolarizing Factor-Mediated Hyperpolarization in the Human Saphenous Vein 
Jian-An Yang, MD, Guo-Wei He, MD, PhD 
The Annals of Thoracic Surgery 
Volume 63, Issue 2, Pages (February 1997)
DOI: /S (96)

2. Fig. 1
Diagram of measuring and recording set-up for membrane potential of human saphenous vein in the organ bath.
The Annals of Thoracic Surgery  , DOI: ( /S (96) )

3. Fig. 2
An actual trace of the membrane potential measurement in a single smooth muscle cell of the human saphenous vein. Concentration–response (membrane potential) curves were established for acetylcholine (ACh) in the presence of indomethacin (7 μmol/L) and NG-nitro-l-arginine (300 μmol/L) in the endothelium-intact (a), endothelium-denuded (b), and surgically prepared (c) human saphenous vein. The numbers refer to −log M concentrations.
The Annals of Thoracic Surgery  , DOI: ( /S (96) )

4. Fig. 3
Concentration-dependent curves of membrane potential by acetylcholine (−9 to −5 log M) in normal human saphenous vein (control group). Vertical bars are 1 standard error of the mean of the response at each concentration. (Solid circle = control; open circles = in the presence of NG-nitro-l-arginine [LNNA, 300 μmol/L] and indomethacin [INDO, 7 μmol/L]; triangles = endothelium-denuded preparation [E−].)
The Annals of Thoracic Surgery  , DOI: ( /S (96) )

5. Fig. 4
Concentration-dependent curves of membrane potential by acetylcholine (−9 to −5 log M) in the human saphenous vein undergoing surgical preparation (surgical preparation group). Vertical barsare 1 standard error of the mean of the response at each concentration. (Closed circles = control; open circles = human saphenous vein undergoing surgical preparation; triangles = endothelium-denuded preparation [E−]; ∗p < 0.05 compared with the control.)
The Annals of Thoracic Surgery  , DOI: ( /S (96) )