1. Volume 64, Pages S21-S26 (October 2003)
Renal expression of angiotensin type 2 (AT2) receptors during kidney damage 
Marta Ruiz-Ortega, Vanesa Esteban, Yusuke Suzuki, Mónica Ruperez, Sergio Mezzano, Leopoldo Ardiles, Pilar Justo, Alberto Ortiz, Jesús Egido 
Kidney International 
Volume 64, Pages S21-S26 (October 2003)
DOI: /j s86.5.x
Copyright © 2003 International Society of Nephrology Terms and Conditions

2. Figure 1
Systemic infusion of angiotensin II (Ang II) into rats up-regulates renal AT2 expression. Immunohistochemistry was done as described in Methods. In control rats, AT2 is present in low levels in some tubular cells. In Ang II–infused rats for 3 days AT2 is over-expressed in tubular cells and appeared in glomeruli. Negative control samples were incubated in the absence of primary antibody.
Kidney International  , S21-S26DOI: ( /j s86.5.x)
Copyright © 2003 International Society of Nephrology Terms and Conditions

3. Figure 2
Systemic infusion of angiotensin II (Ang II) into mice up-regulates tubular AT2 expression. In Ang II–infused mice AT2 expression was elevated in tubular cells compared to control mice. Similar results were found in AT1 knockout mice.
Kidney International  , S21-S26DOI: ( /j s86.5.x)
Copyright © 2003 International Society of Nephrology Terms and Conditions

4. Figure 3
Analysis of AT2 mRNA expression by reverse transcription-polymerase chain reaction (RT-PCR) in murine models of renal damage. Total RNA from renal cortex of control mice expressed AT2 mRNA. In both models—infusion of (A) Ang II and injection of (B) folic acid—AT2 mRNA expression was increased compared to control animals.
Kidney International  , S21-S26DOI: ( /j s86.5.x)
Copyright © 2003 International Society of Nephrology Terms and Conditions

5. Figure 4
Angiotensin II receptor type 2 (AT2) is up-regulated in folic acid–induced renal injury. In control Balb-c mice, a weak staining for AT2 in tubular cells was observed. Injection of folic acid up-regulated tubular AT2 expression.
Kidney International  , S21-S26DOI: ( /j s86.5.x)
Copyright © 2003 International Society of Nephrology Terms and Conditions

6. Figure 5
(A) Immunohistochemical expression of AT1 and AT2 receptors in control animals and rats with overload nephropathy. (B) Image analyzer quantification of immunohistochemical expression of AT1 and AT2. White bars represent AT1 and black bars represent AT2. P < 0.05 compared with AT1.
Kidney International  , S21-S26DOI: ( /j s86.5.x)
Copyright © 2003 International Society of Nephrology Terms and Conditions