1. Chromatography Chromatography
means of separating and tentatively identifying the components of a mixture.

2. Chromatography
Molecules that have a preference for the mobile phase will slowly pull ahead and separate from those substances that prefer to remain in the stationary phase.

3. An analogy

4. Paper Chromatography

5. Rf Values

6. Calculate the Rf value for each spot

7. TLC
TLC uses a solid stationary phase usually coated onto a glass plate and a mobile liquid phase to separate the components of the mixture.

8. TLC The liquid will slowly rise up the plate by capillary action
the sample to become distributed between the stationary phase and the moblie liquid phase.
Most compounds are colorless
materials must be visualized by placing the plates under ultraviolet light or spraying the plate with a chemical reagent.
Rf values calculated.