1. Volume 125, Issue 5, Pages 1452-1461 (November 2003)
Mesenteric vasoconstriction triggers nitric oxide overproduction in the superior mesenteric artery of portal hypertensive rats 
Ming-Hung Tsai, Yasuko Iwakiri, Gregory Cadelina, William C Sessa, Roberto J Groszmann 
Gastroenterology 
Volume 125, Issue 5, Pages (November 2003)
DOI: /j.gastro

2. Figure 1
The perfusion pressure changes in response to methoxamine in isolated mesenteric arterial beds at different times. (A) The vasopressor responses of the PVL-20G animals were compared with the sham-operated animals at different times. n = 5–8 per group per each time point. ∗P < 0.01, #P < compared with the corresponding sham rats. (B) The vasopressor responses to methoxamine after NOS inhibitor (l-NMMA) treatment in the isolated SMA beds. n = 4–6 per group per each time point. MTx30, methoxamine 30 μmol/L; MTx100, 100 μmol/L.
Gastroenterology  , DOI: ( /j.gastro )

3. Figure 2
Total NOS activity in rat SMA at different times. NOS activity was significantly enhanced in the SMA of the PVL-20G rats after 10 hours compared with the sham-operated rats. n = 3–6 per group per each time point. ∗P < 0.05 compared with corresponding sham-operated rats.
Gastroenterology  , DOI: ( /j.gastro )

4. Figure 3
The perfusion pressure changes in response to methoxamine in isolated mesenteric arteries. (A) The comparison of contractile responses with methoxamine in the isolated mesenteric arterial beds of PVL-20G, PVL-18G, and sham rats. MTx30, methoxamine 30 μmol/L; MTx100, 100 μmol/L. n = 6 for sham, n = 8 for PVL-20G, and n = 6 for PVL-18G. ∗P < 0.05 compared with sham; ∗∗P < 0.05 compared with PVL-18G. (B) The comparison of contractile responses to methoxamine in mesenteric arterial beds isolated from the RAL and sham rats (n = 7 in each group; ∗P < 0.01). The SMA beds were isolated at 10 hours after RAL or sham operation. (C) Effects of NOS inhibition by l-NMMA on the contractile response to methoxamine in isolated mesenteric arterial beds. Preincubation with l-NMMA abolished the differences in the pressure response to methoxamine between the PVL-20G (n = 4) and sham (n = 5) groups. (D) Preincubation with l-NMMA abolished the differences in the pressure response to methoxamine between the RAL (n = 5) and sham (n = 4) groups.
Gastroenterology  , DOI: ( /j.gastro )

5. Figure 4
Total NOS activity in homogenized SMA beds. (A) Total NOS activity in the SMA of the PVL (n = 5 for PVL-20G; n = 4 for PVL-18G) and the sham (n = 5) groups. ∗P < Total NOS activity was significantly enhanced in the SMA of PVL-20G rats compared with PVL-18G and sham rats. (B) Total NOS activity in the SMA isolated 10 hours after RAL (n = 6) and sham (n = 6) operation. RAL exhibited significantly higher NOS activity compared with the sham rats. ∗P < 0.05.
Gastroenterology  , DOI: ( /j.gastro )

6. Figure 5
eNOS and iNOS protein expression in SMA beds. (A) eNOS and iNOS protein expression in the SMA of the PVL (n = 4 for PVL-18G; n = 3 for PVL-20G) and the sham (n = 3) groups. Bovine aorta endothelial cell lysate was used as a positive control for eNOS. Lysate of peritoneal macrophages stimulated with lipopolysaccharide 100 ng/mL for 16 hours was used for the positive control for iNOS protein. (B) eNOS and iNOS protein expression in renal artery harvested 10 hours after RAL (n = 3) and sham (n = 3) operation. There was no significant difference in SMA eNOS expression in PVL-20G, PVL-18G, and RAL rats compared with the corresponding sham-operated rats.
Gastroenterology  , DOI: ( /j.gastro )

7. Figure 6
Hemodynamic characteristics of PVL-20G, PVL-18G, and RAL and the sham-operated rats. (A) Hemodynamic study of the PVL and sham groups. PP, portal pressure; QSMA, SMA blood flow. ∗P < 0.05 compared with the sham group. Sham, n = 5; PVL-20G, n = 6; PVL-18G, n = 5. (B) Hemodynamic study of the RAL and sham groups. ∗P < Sham, n = 6; RAL, n = 5. One-way analysis of variance followed by Scheffe test.
Gastroenterology  , DOI: ( /j.gastro )