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Amino acid sequence alignment of beetle luciferases.
Amino acid sequence alignment of beetle luciferases. The amino acid sequence alignment of loop348–361 and loop521–528 in REPh and loop351–364 and loop523–530 in GBAv was performed against luciferases from P. pyralis (GPp), Luciola cruciate (GLc), and Lampyris turkestanicus using Clustal Omega. The loop regions are highlighted in yellow. Amino acids R337 and K524 that altered the emission color of GBAv are boxed. Amino acid R353 insertion in REPh is indicated with an arrow that is also found in L. turkestanicus luciferase. César Carrasco-López et al. LSA 2018;1:e © 2018 Carrasco-López et al.
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