1. A
n.s. WT
4-1BB-/- B
n.s. WT
4-1BB-/-
Supplemental figure 2: Proliferation of 4-1BB-/- CD8+ T cells is not globally affected by the loss of 4-1BB. Purified CD8+ T cells (0.2×106 cells/well) were activated with plate bound anti-CD3ε (0.5ug/ml) and (A) soluble anti-CD28 (1ug/ml) in a tissue culture treated round bottom plate or (B) plate bound anti-OX-40 (5ug/ml) in a high binding flat bottom plate. Proliferation was assessed using 3H-thymidine uptake 72 hours post activation. Statistics were generated by student t test, n.s.; not significant when comparing groups indicated by horizontal lines.