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Role of the T-cell receptor in kidney ischemia–reperfusion injury

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Presentation on theme: "Role of the T-cell receptor in kidney ischemia–reperfusion injury"— Presentation transcript:

1 Role of the T-cell receptor in kidney ischemia–reperfusion injury
V. Savransky, R.R. Molls, M. Burne-Taney, C.-C. Chien, L. Racusen, H. Rabb  Kidney International  Volume 69, Issue 2, Pages (January 2006) DOI: /sj.ki Copyright © 2006 International Society of Nephrology Terms and Conditions

2 Figure 1 Survival of TCR-deficient mice and WT mice at 24 h after IRI (n=12 in each group). Kidney International  , DOI: ( /sj.ki ) Copyright © 2006 International Society of Nephrology Terms and Conditions

3 Figure 2 Renal function 24 h after IRI in TCR αβ-, γδ-deficient, and WT mice (n=12 in each group). *Significant difference between WT and TCR αβ-deficient mice; P<0.05. Kidney International  , DOI: ( /sj.ki ) Copyright © 2006 International Society of Nephrology Terms and Conditions

4 Figure 3 Flow cytometry analysis confirms the deficiency of TCR αβ and γδ CD3+ cells in TCR αβ- and γδ-deficient mice, respectively. Kidney International  , DOI: ( /sj.ki ) Copyright © 2006 International Society of Nephrology Terms and Conditions

5 Figure 4 Histopathology of postischemic kidney tissue by H&E, original magnification × 64 in (a) sham, (b) WT, (c) TCR αβ-, and (d) γδ-deficient mice at 24 h postischemia. Kidney International  , DOI: ( /sj.ki ) Copyright © 2006 International Society of Nephrology Terms and Conditions

6 Figure 5 Tubular injury scoring of kidney at 24 h postischemia in WT, TCR αβ-, and γδ-deficient mice. CT: convoluted tubule, SS: straight segment, OS: outer stripe, IS: inner stripe. Kidney International  , DOI: ( /sj.ki ) Copyright © 2006 International Society of Nephrology Terms and Conditions

7 Figure 6 Neutrophil and macrophage infiltration into the post ischemic kidney using the MPO assay. No significant difference between WT, TCR αβ, and γδ deficient mice at 24 h post ischemia. Kidney International  , DOI: ( /sj.ki ) Copyright © 2006 International Society of Nephrology Terms and Conditions

8 Figure 7 Proinflammatory cytokine and chemokine levels (pg/ml) at 24 h after IRI in kidney tissue homogenate. (a) TNF-α, (b) IL-6, (c) IL-1β, (d) IL-2, (e) IFN-γ, and (f) RANTES. *Significant difference between WT and TCR αβ-deficient mice; P<0.05. Kidney International  , DOI: ( /sj.ki ) Copyright © 2006 International Society of Nephrology Terms and Conditions


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