Presentation is loading. Please wait.

Presentation is loading. Please wait.

Solar-Simulated Ultraviolet Radiation Induces Abnormal Maturation and Defective Chemotaxis of Dendritic Cells  María Mittelbrunn, Reyes Tejedor, Hortensia.

Published byLinnéa Fredriksson Modified over 5 years ago

Similar presentations

Presentation on theme: "Solar-Simulated Ultraviolet Radiation Induces Abnormal Maturation and Defective Chemotaxis of Dendritic Cells  María Mittelbrunn, Reyes Tejedor, Hortensia."— Presentation transcript:

1 Solar-Simulated Ultraviolet Radiation Induces Abnormal Maturation and Defective Chemotaxis of Dendritic Cells  María Mittelbrunn, Reyes Tejedor, Hortensia de la Fuente, M. Ángeles García-López, Ángeles Ursa, Pablo F. Peñas, Amaro García- Díez, José Luis Alonso-Lebrero, Juan Pablo Pivel, Salvador González, Roberto Gonzalez-Amaro, Francisco Sánchez-Madrid  Journal of Investigative Dermatology  Volume 125, Issue 2, Pages (August 2005) DOI: /j X x Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Phenotype of human monocyte-derived dendritic cells (DC) and Langerhans cells (LC). Human monocytes were induced to differentiate to immature DC, and Langerhans-like DC for 6 d with granulocyte macrophage colony simulating factor/interleukin-4 (GM-CSF/IL-4) or with GM-CSF/IL-4 and transformation growth factor-β, respectively. The phenotype of immature (thin line) and mature (lipopolysaccharide treatment for 20 h, solid line) DC and LC was analyzed by flow cytometry. Dotted line corresponds to the negative control. Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 Solar-simulated ultraviolet (UV) radiation spectrum. UV spectrum emission of the solar simulator used in this study. The sunlight spectrum determined by the DIN standard is also shown. Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 Induction of a partial mature dendritic cell (DC) phenotype by solar-simulated ultraviolet (UV) radiation. (A) Human immature monocyte-derived DC (open bars), induced to maturate with lipopolysaccharide 10 ng per mL) for 20 h (striped bars), or 20 h after exposure to solar-simulated UV radiation (5.0 J per cm2 UVA+0.4 J per cm2 UVB) (solid bars) were analyzed for the surface expression of DC-SIGN, mannose receptor, major histocompatibility complex-II, CD86, and CD83 by flow cytometry. Data represent the arithmetic mean±SEM of fluorescence intensity of 10 different experiments *(p<0.05 compared with immature DC or indicated). (B) Langerhans-like DC were treated and analyzed as in A. Data represent the arithmetic mean±SEM of fluorescence intensity of three different experiments. ManR: mannose receptor C. Endocytic activity of immature (open bars), mature (striped bars), or irradiated (solid bars) DC was analyzed. Cells were incubated with fluorescein isothiocyanate–dextran for 30 min at 0°C (negative control) or at 37°C and fluorescence capture was analyzed by flow cytometry. Data represent the arithmetic mean±SEM of fluorescence intensity of six different experiments.* (p<0.05 compared with immature DC). Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 Dose–response and time course of costimulatory/antigen receptor expression in dendritic cells (DC) after solar simulated ultraviolet (UV) radiation. (A) Immature human monocyte-derived DC were exposed to different doses of solar-simulated UV radiation. DC-SIGN, major histocompatibility complex (MHC)-II, and CD86 levels were analyzed 20 h after irradiation. (B) Time course of costimulatory/antigen receptors expression in DC after 5.0 J per cm2 UVA+0.4 J per cm2 UVB radiation. Data represent the arithmetic mean of fluorescence intensity of one representative experiment of three. Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

6 Figure 5 Irradiated dendritic cells (DC) are transiently refractory to a potent maturation stimulus. (A) Irradiated and non-irradiated DC were incubated for 20 h in the absence (open bars) or presence of lipopolysaccharides (LPS) (10 ng per mL) (solid bars). Expression levels of CD83, CD86, major histocompatibility complex (MHC)-II and DC-SIGN were analyzed by flow cytometry analysis. Data represent the arithmetic mean±SEM of fluorescence intensity of five different experiments *(p<0.05 compared with cells cultured in the absence of LPS). (B) Kinetics analysis of CD86 expression in DC after 5.0 J per cm2 ultraviolet (UV)A+0.4 J per cm2 UVB radiation in the presence or absence of LPS. Data from a representative experiment out of three performed are shown. Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

7 Figure 6 Effect of ultraviolet (UV) radiation on the T cell stimulatory capacity of dendritic cells (DC). DC were irradiated with a solar simulator (5.0 J per cm2 UVA+0.4 J per cm2 UVB) and then incubated in the presence or absence of lipopolysaccharides (LPS). After 30 h, DC were used to stimulate allogenic CD4+T lymphocytes in 96-well plates, and cell proliferation was measured by 3H-TdR incorporation, as stated in Materials and Methods. Data from a representative experiment out of three performed are shown. Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

8 Figure 7 Cytokine secretion by dendritic cell (DC) after solar-simulated ultraviolet (UV) radiation. (A, C, D) Human immature DC were irradiated or not with 5.0 J per cm2 UVA+0.4 J per cm2 UVB, and culture for 20 h in the absence or presence of lipopolysaccharide (LPS) 10 ng per mL). Levels of interleukin (IL)-10, p70 IL-12, or tumor necrosis factor (TNF)-α in cultured supernatants were detected by ELISA. (B) Immature DC were exposed to different doses of solar-simulated UV radiation. TNF-α in the supernatant was measured by ELISA 20 h after irradiation. The arithmetic mean±SEM of six different experiments is shown. Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

9 Figure 8 Induction of dendritic cell (DC) apoptosis by solar-simulated irradiation. Human immature DC were irradiated with different doses of solar-simulated ultraviolet (UV) radiation. Apoptotic cells were detected, 24 h later, by two different approaches. (A) Cells were analyzed for their DNA content by flow cytometry. Results represent the percentage of hypodiploid (apoptotic) cells from four different experiments. The arithmetic mean±SEM is shown. (B) Cells were incubated with fluorescein isothiocyanate–Annexin V and analyzed by flow cytometry. Results represent the percentage of positive (apoptotic) cells from four different experiments. The arithmetic mean±SEM is shown. Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

10 Figure 9 Solar-simulated radiation induces dendritic cell (DC) expression of CXCR4, but not CCR7 and chemotaxis to stromal cell-derived factor 1(SDF-1), but not SLC. (A) Human immature monocyte-derived DC, induced to mature with lipopolysaccharide (LPS) 10 ng per mL) for 20 h, or 20 h after exposition to solar-simulated ultraviolet (UV) radiation (5.0 J per cm2 UVA+0.4 J per cm2 UVB) were analyzed for the surface expression CXCR4 (open bars), CCR1 (gray bars), and CCR7 (solid bars) by flow cytometry. Data represent the arithmetic mean±SEM of positive cells of seven different experiments. *(p<0.05 compared with immature DC). (B) Immature, mature, or irradiated DC were allowed to migrate as described in Materials and Methods. Data correspond to the arithmetic mean±SEM of the percentage of migrating cells of four independent experiments. (C) Irradiated and non-irradiated DC were incubated for 20 h in the absence (open bars) or presence of LPS (10 ng per mL) (solid bars). Then, the expression of CCR7 was analyzed by flow cytometry. Data correspond to the arithmetic mean±SEM of the percent-positive cells of eight different experiments. *(p<0.05 compared with immature DC or indicated). A representative histogram of CCR7 expression of DC upon UV radiation is also shown. (D) Kinetics analysis of CCR7 expression in DC after 5.0 J per cm2 UVA+0.4 J per cm2 UVB radiation in the presence or absence of LPS. Data represent the arithmetic mean of fluorescence intensity of one representative experiment out of three. Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Download ppt "Solar-Simulated Ultraviolet Radiation Induces Abnormal Maturation and Defective Chemotaxis of Dendritic Cells  María Mittelbrunn, Reyes Tejedor, Hortensia."

Similar presentations

Dynamic Changes in Resident and Infiltrating Epidermal Dendritic Cells in Active and Resolved Psoriasis  Elisa Martini, Maria Wikén, Stanley Cheuk, Irène.

Dynamic Changes in Resident and Infiltrating Epidermal Dendritic Cells in Active and Resolved Psoriasis  Elisa Martini, Maria Wikén, Stanley Cheuk, Irène.
Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites 

Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites 
In situ Delivery of Tumor Antigen– and Adjuvant-Loaded Liposomes Boosts Antigen- Specific T-Cell Responses by Human Dermal Dendritic Cells  Martine A.

In situ Delivery of Tumor Antigen– and Adjuvant-Loaded Liposomes Boosts Antigen- Specific T-Cell Responses by Human Dermal Dendritic Cells  Martine A.
Dermal CD14+ Dendritic Cell and Macrophage Infection by Dengue Virus Is Stimulated by Interleukin-4  Evelyne Schaeffer, Vincent Flacher, Vasiliki Papageorgiou,

Dermal CD14+ Dendritic Cell and Macrophage Infection by Dengue Virus Is Stimulated by Interleukin-4  Evelyne Schaeffer, Vincent Flacher, Vasiliki Papageorgiou,
UV Radiation Induces the Epidermal Recruitment of Dendritic Cells that Compensate for the Depletion of Langerhans Cells in Human Skin  Amine Achachi,

UV Radiation Induces the Epidermal Recruitment of Dendritic Cells that Compensate for the Depletion of Langerhans Cells in Human Skin  Amine Achachi,
Μ-Opioid Receptor Is Induced by IL-13 within Lymph Nodes from Patients with Sézary Syndrome  Alan Bénard, Pierre Cavaillès, Jérôme Boué, Emmanuelle Chapey,

Μ-Opioid Receptor Is Induced by IL-13 within Lymph Nodes from Patients with Sézary Syndrome  Alan Bénard, Pierre Cavaillès, Jérôme Boué, Emmanuelle Chapey,
Activators of Peroxisome Proliferator-Activated Receptors Protect Human Skin from Ultraviolet-B-Light-Induced Inflammation  Stefan Kippenberger, Marcella.

Activators of Peroxisome Proliferator-Activated Receptors Protect Human Skin from Ultraviolet-B-Light-Induced Inflammation  Stefan Kippenberger, Marcella.
Sphingosylphosphorylcholine is a Potent Inducer of Intercellular Adhesion Molecule-1 Expression in Human Keratinocytes  Genji Imokawa, Yutaka Takagi,

Sphingosylphosphorylcholine is a Potent Inducer of Intercellular Adhesion Molecule-1 Expression in Human Keratinocytes  Genji Imokawa, Yutaka Takagi,
Prostaglandin E2 is a key factor for CCR7 surface expression and migration of monocyte-derived dendritic cells by Elke Scandella, Ying Men, Silke Gillessen,

Prostaglandin E2 is a key factor for CCR7 surface expression and migration of monocyte-derived dendritic cells by Elke Scandella, Ying Men, Silke Gillessen,
Corticosteroids But Not Pimecrolimus Affect Viability, Maturation and Immune Function of Murine Epidermal Langerhans Cells  Wolfram Hoetzenecker, Josef.

Corticosteroids But Not Pimecrolimus Affect Viability, Maturation and Immune Function of Murine Epidermal Langerhans Cells  Wolfram Hoetzenecker, Josef.
Cord Blood CD34+ Cells Differentiate into Dermal Dendritic Cells in Co-Culture with Cutaneous Fibroblasts or Stromal Cells  Zia U.A. Mollah, Setsuya Aiba,

Cord Blood CD34+ Cells Differentiate into Dermal Dendritic Cells in Co-Culture with Cutaneous Fibroblasts or Stromal Cells  Zia U.A. Mollah, Setsuya Aiba,
IC3b Arrests Monocytic Cell Differentiation Into CD1c-Expressing Dendritic Cell Precursors: A Mechanism for Transiently Decreased Dendritic Cells in vivo.

IC3b Arrests Monocytic Cell Differentiation Into CD1c-Expressing Dendritic Cell Precursors: A Mechanism for Transiently Decreased Dendritic Cells in vivo.
IL-13-Stimulated Human Keratinocytes Preferentially Attract CD4+CCR4+ T cells: Possible Role in Atopic Dermatitis  Rahul Purwar, Thomas Werfel, Miriam.

IL-13-Stimulated Human Keratinocytes Preferentially Attract CD4+CCR4+ T cells: Possible Role in Atopic Dermatitis  Rahul Purwar, Thomas Werfel, Miriam.
The Late Endosomal Adaptor Molecule p14 (LAMTOR2) Regulates TGFβ1-Mediated Homeostasis of Langerhans Cells  Florian Sparber, Christoph H. Tripp, Kerstin.

The Late Endosomal Adaptor Molecule p14 (LAMTOR2) Regulates TGFβ1-Mediated Homeostasis of Langerhans Cells  Florian Sparber, Christoph H. Tripp, Kerstin.
CD69 Modulates Sphingosine-1-Phosphate-Induced Migration of Skin Dendritic Cells  Amalia Lamana, Pilar Martin, Hortensia de la Fuente, Laura Martinez-Muñoz,

CD69 Modulates Sphingosine-1-Phosphate-Induced Migration of Skin Dendritic Cells  Amalia Lamana, Pilar Martin, Hortensia de la Fuente, Laura Martinez-Muñoz,
Age-Related Differences in Sensitivity of Peripheral Blood Monocytes to Lipopolysaccharide and Staphylococcus Aureus Toxin B in Atopic Dermatitis  Marie.

Age-Related Differences in Sensitivity of Peripheral Blood Monocytes to Lipopolysaccharide and Staphylococcus Aureus Toxin B in Atopic Dermatitis  Marie.
TGFβ Induces a SAMHD1-Independent Post-Entry Restriction to HIV-1 Infection of Human Epithelial Langerhans Cells  Magdalena A. Czubala, Katja Finsterbusch,

TGFβ Induces a SAMHD1-Independent Post-Entry Restriction to HIV-1 Infection of Human Epithelial Langerhans Cells  Magdalena A. Czubala, Katja Finsterbusch,
Interleukin-3Rα+ Myeloid Dendritic Cells and Mast Cells Develop Simultaneously from Different Bone Marrow Precursors in Cultures with Interleukin-3  Thomas.

Interleukin-3Rα+ Myeloid Dendritic Cells and Mast Cells Develop Simultaneously from Different Bone Marrow Precursors in Cultures with Interleukin-3  Thomas.
Norito Katoh, Fujiko Soga, Takeshi Nara, Koji Masuda, Saburo Kishimoto 

Norito Katoh, Fujiko Soga, Takeshi Nara, Koji Masuda, Saburo Kishimoto 
Granulocyte–Macrophage Colony-Stimulating Factor Gene Transfer to Dendritic Cells or Epidermal Cells Augments Their Antigen-Presenting Function Including.

Granulocyte–Macrophage Colony-Stimulating Factor Gene Transfer to Dendritic Cells or Epidermal Cells Augments Their Antigen-Presenting Function Including.

Similar presentations

Ads by Google