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The impact of psychogenic stressors on oxidative stress markers and patterns of CYP2E1 expression in mice liver  Oksana Maksymchuk, Mykola Chashchyn 

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Presentation on theme: "The impact of psychogenic stressors on oxidative stress markers and patterns of CYP2E1 expression in mice liver  Oksana Maksymchuk, Mykola Chashchyn "— Presentation transcript:

1 The impact of psychogenic stressors on oxidative stress markers and patterns of CYP2E1 expression in mice liver  Oksana Maksymchuk, Mykola Chashchyn  Pathophysiology  Volume 19, Issue 3, Pages (June 2012) DOI: /j.pathophys Copyright © 2012 Elsevier Ireland Ltd Terms and Conditions

2 Fig. 1 CYP2E1 protein expression in mice liver under chronic psychogenic stress. (A) Western blot analysis of total liver lysates probed by specific anti-CYP2E1 antibodies is presented. Tubulin is a loading control. (B) The quantification of Western-blotting result. 1 – control; 2 – stressor's effect within 7 days, analyzed immediately after stressor termination; 3 – stressor's effect within 14 days, analyzed immediately after stressor termination; 4 – stressor's effect within 14 days, analyzed on the 7th day after the stressor termination. * P<0.05 compared to control (n=4–5). Pathophysiology  , DOI: ( /j.pathophys ) Copyright © 2012 Elsevier Ireland Ltd Terms and Conditions

3 Fig. 2 CYP2E1 mRNA expression in mice liver under the chronic effect of psychogenic stressors detected by RT-RCR analysis (A). (B) The quantification of Cyp2e1 mRNA expression (Cyp2e1 values were normalized by β-actin mRNA value): 1 – control; 2 – stressor's effect within 7 days; 3 – stressor's effect within 14 days; 4 – stressor's effect within 14 days and analyzed on the 7th day after the stressor termination. Pathophysiology  , DOI: ( /j.pathophys ) Copyright © 2012 Elsevier Ireland Ltd Terms and Conditions


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