1. Cytosolic entry of Lm required for CD8α+ DC recruitment.
Cytosolic entry of Lm required for CD8α+ DC recruitment. (A) Confocal microscopy of spleen sections from WT mice at 12 hpi with WT Lm (top), ActA knockout (KO) Lm (middle), and LLO KO Lm (bottom). Lm are rendered with spots in green. CD169 is shown in blue, and colocalization of CD11c and CD8α is shown in red. Scale bars, 50 μm. (B) Quantification of intracellular Lm within CD169+ macrophages at 12 hpi. Each symbol represents a different confocal image quantified. All data are representative of two separate experiments with two to three mice per group.
Oriana A. Perez et al. Sci. Immunol. 2017;2:eaah5520
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