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Features of follicle-stimulating hormone–stimulated follicles in a sheep model: keys to elucidate embryo failure in assisted reproductive technique cycles 

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Presentation on theme: "Features of follicle-stimulating hormone–stimulated follicles in a sheep model: keys to elucidate embryo failure in assisted reproductive technique cycles "— Presentation transcript:

1 Features of follicle-stimulating hormone–stimulated follicles in a sheep model: keys to elucidate embryo failure in assisted reproductive technique cycles  Almudena Veiga-Lopez, Ph.D., Veronica Dominguez, B.Sc., Carlos J.H. Souza, Ph.D., Rosa M. Garcia-Garcia, Ph.D., Carmen Ariznavarreta, Ph.D., Jesus A.F. Tresguerres, Ph.D., Alan S. McNeilly, Ph.D., Antonio Gonzalez-Bulnes, Ph.D.  Fertility and Sterility  Volume 89, Issue 5, Pages (May 2008) DOI: /j.fertnstert Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

2 Figure 1 Schematic representation of the treatment cycle and the sampling protocol. PG, Prog, and FSH = administration of prostaglandin analogues, progestagens, and FSH, respectively. OVX = ovariectomy. Ultrasonographies of ovaries were performed coincidentally with encircled FSH. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

3 Figure 2 Mean (±SEM) concentrations (continuous lines) of inhibin A (open circles) and estradiol (filled circles) from 0 to 84 hours after the start of the exogenous FSH treatment. Mean number of follicles (dotted lines) sized ≥2 mm (open circles) and ≥4 mm (filled circles) from 0 to 60 hours after the start of the exogenous FSH treatment. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

4 Figure 3 Mean intrafollicular concentrations for inhibin A (A, μg/mL), estradiol (B, ng/mL), and androstenedione (C, ng/mL) and for the estradiol–androstenedione ratio (D) of preovulatory follicles (diameter, 4–8 mm), dissected at detection of estrus behavior. The follicle size is represented by the X axis; the first number represents the mean concentration for each follicular size, the second number is the number of follicles (N) evaluated for each size, and the bubble size represents the N relative to the total. The SEMs have been omitted for clarity. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

5 Figure 4 Mean (±SEM) number of newly recruited, growing, and atretic follicles from 0 to 60 hours after the start of the exogenous FSH treatment in sheep bearing (filled circles) or not (open circles) a follicle sized ≥6 mm at 0 and 12 hours. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

6 Figure 5 Mean (±SEM) plasma estradiol concentration from 0 to 60 hours after the start of the exogenous FSH treatment in sheep bearing (filled circles) or not (open circles) a follicle sized ≥6 mm at 0 and 12 hours. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions


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