1. Supplemental Figure 4 A
no agonist
MALP2
PAM3CSK4
GFP WT F217S
GFP WT F217S
GFP WT F217S
p-IRAK1 (T209)
IRAK1 B
GFP WT
F217S C
GFP
GFP WT WT
F217S
F217S
Figure S4. TLR2-F217 and downstream signals. (a) Western blot characterization of IRAK1 phospho-T209 in 293T cells transfected with either WT or F217S TLR2 and treated with no agonist, MALP2 or PAM3CSK4. Top, Western blot analysis of p-T209 of IRAK1; Bottom, Western blot analysis of IRAK1. (b) Q-PCR analysis of IL8-RB gene expression in 293T with indicated transfection and agonist treatments. (b) Q-PCR analysis of IL8 and JMJD3 gene expression in OCI-AML3 cells with indicated transfection and agonist treatments.