1. Volume 65, Issue 5, Pages 1897-1905 (May 2004)
Local overexpression of C-type natriuretic peptide ameliorates vascular adaptation of porcine hemodialysis grafts 
Joris I. Rotmans, Hence J.M. Verhagen, Evelyn Velema, Dominique P.V. de Kleijn, Mieke van den Heuvel, John J.P. Kastelein, Gerard Pasterkamp, Erik S.G. Stroes 
Kidney International 
Volume 65, Issue 5, Pages (May 2004)
DOI: /j x
Copyright © 2004 International Society of Nephrology Terms and Conditions

2. Figure 1
Experimental protocol. The amount of virus applied to the jugular vein is expressed in infectious units (IU). Abbreviations are: AV, arteriovenous; Ad.CNP, adenoviral vector encoding murine C-type natriuretic peptide; β-gal, β-galactosidase; RT-PCR, reverse transcription-polymerase chain reaction; RIA, radioimmunoassay.
Kidney International  , DOI: ( /j x)
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3. Figure 2
Gene expression 3days after transduction with adenoviral vector encoding murine C-type natriuretic peptide (Ad.CNP) or Ad.mock, analyzed by reverse transcription-polymerase chain reaction (RT-PCR). Left panel shows gene expression in cultured porcine fibroblasts. (lanes 1 and 2, Ad.mock-transduced fibroblasts; lanes 3 and 4, Ad.CNP-transduced fibroblasts). Right panel shows the gene expression after in vivo gene transfer (lane 5, Ad.mock-transduced vein; lane 6, Ad.CNP-transduced vein). The amount of β-actin mRNA was used as internal standards.
Kidney International  , DOI: ( /j x)
Copyright © 2004 International Society of Nephrology Terms and Conditions

4. Figure 3
Effect of C-type natriuretic peptide (CNP)-22 and adenoviral vector encoding murine C-type natriuretic peptide (Ad.CNP) transduction on the migration of cultured porcine vein fibroblasts. Values are expressed as the mean percentage ± SEM of migrated cells, as compared with the positive control and Ad.mock, respectively. The negative control represents fibroblast migration without fetal calf serum (FCS) stimulation. *P = 0.05; **P = 0.04.
Kidney International  , DOI: ( /j x)
Copyright © 2004 International Society of Nephrology Terms and Conditions

5. Figure 4
Representative Elastin von Gieson–stained 5 μm section of an adenoviral (Ad.mock)-transduced (A) and adreoviral vector encoding murine C-type natriuretic peptide (Ad.CNP)-transduced (B) vein (original magnification 12.5×). (C) Detail of Ad.LacZ-transduced vein, 3days after transduction (original magnification 100×). (D) Detail of α-smooth muscle cell actin–stained section. The dotted line indicated the boundary of the neointima area (original magnification 200×). The intima predominantly consists of vascular smooth muscle cells (VSMC).
Kidney International  , DOI: ( /j x)
Copyright © 2004 International Society of Nephrology Terms and Conditions