1. Enrichment analysis using DAVID
By NFU Biology Algorithm lab

2. Outline What is DAVID DAVID Tools Tools Demo Functional Annotation
Gene Functional Classification
Gene ID Conversion

3. What is DAVID
DAVID is a Bioinformatics Database, and it is also provides many tools to analysis the gene data.

4. DAVID Tools DAVID Tools provides many function about
1. Functional Annotation
2. Gene Function Classification
3. Gene ID Conversion
4. Gene Name Batch Viewer
5. NIAID Pathogen Annotation

5. Tools Demo Functional Annotation
Step1:Upload the Gene list for DAVID from Start Analysis

6. Tools Demo Step2&3: Upload and select list type
Upload list or file to DAVID
Select Identifier and Gene List

7. Tools Demo

8. Tools Demo The gene list number from users upload
Choice what kind tools you want
(ex: Functional Annotation Tool)
Upload list file name

9. Tools Demo Annotation Summary Results
Individual view report:Percentage (involved genes/total genes)Gene from your list Single Chart Report for ONLY this annotation category
View and select annotation categories of your interests.
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10. Tools Demo Annotation Summary Results
建議如下： Select Gene-ontology (BP_1) pathways (5)

11. Tools Demo Functional Annotation Clustering
Clustering options and stringency
The overall enrichment score for the group based on the EASE score of each term members.
The higher, the more enriched.

12. Tools Demo Functional Annotation Chart
Minimum number of genes for the corresponding term.
Maximum EASE Score/P-value
Modified Fisher Exact P-value, EASE Score. The smaller, the more enriched.
Related term search
Original database/resource where the terms orient
Enriched terms associated with your gene list

13. Tools Demo Functional Annotation Table
Gene Name
Annotation terms

14. Tools Demo Gene Functional Classification Result

15. Tools Demo Gene Functional Classification Result
Group options and stringency
Group number
The higher, the more enriched.

16. Tools Demo Gene ID Conversion

17. Tools Demo Gene ID Conversion
選擇想要轉換項目

18. Tools Demo Gene ID Conversion
使用者上傳的內容
轉換結果

19. Thanks For Watching
Producer by National Formosa University Biology Algorithm lab at 2012/12