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Corticoids synergize with IL-1 in the induction of LCN2

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1 Corticoids synergize with IL-1 in the induction of LCN2
J. Conde, V. Lazzaro, M. Scotece, V. Abella, R. Villar, V. López, M.Á. Gonzalez-Gay, J. Pino, R. Gómez, A. Mera, O. Gualillo  Osteoarthritis and Cartilage  Volume 25, Issue 7, Pages (July 2017) DOI: /j.joca Copyright © 2017 Osteoarthritis Research Society International Terms and Conditions

2 Fig. 1 mLCN2 mRNA expression determined by RT-qPCR. ∗indicates P < 0.05; ∗∗indicates P < 0.01 and ∗∗∗indicates P < 0.001 vs unstimulated control. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2017 Osteoarthritis Research Society International Terms and Conditions

3 Fig. 2 mLCN2 protein expression determined by western blot analysis. A, B low panels. Data showing densitometric analysis of all performed western blots. C, D. mMMP-13 expression determined by RT-qPCR. ∗∗indicates P < 0.01 and ∗∗∗indicates P < 0.001 vs unstimulated control. #indicates P < 0.05; ##indicates P < 0.01 and ###indicates P < 0.001 vs IL-1α (0.1 ng/mL). Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2017 Osteoarthritis Research Society International Terms and Conditions

4 Fig. 3 mLCN2 protein expression determined by western blot analysis. A, B low panels. Data showing densitometric analysis of all performed western blots. ∗∗indicates P < 0.01 and ∗∗∗indicates P < 0.001 vs unstimulated control. ##indicates P < 0.01 and ###indicates P < 0.001 vs PRED or ALD (1 μM) plus IL-1α (0.1 ng/mL). Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2017 Osteoarthritis Research Society International Terms and Conditions

5 Fig. 4 A, B mLCN2 protein expression determined by western blot analysis. A, B low panels. Data showing densitometric analysis of all performed western blots. ∗indicates P < 0.05; ∗∗indicates P < 0.01 and ∗∗∗indicates P < 0.001 vs unstimulated control. ##indicates P < 0.01 and ###indicates P < 0.001 vs PRED or ALD (1 μM) plus IL-1α (0.1 ng/mL). C, D, E. mLCN2 mRNA expression determined by RT-qPCR. ∗indicates P < 0.05; ∗∗indicates P < 0.01 and ∗∗∗indicates P < 0.001 vs IL-1α, PRED or ALD. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2017 Osteoarthritis Research Society International Terms and Conditions


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