1. And info you should know about them….
Lab Practical Slides
And info you should know about them….

2. Slide #1 - Paramecium (@ 100X) – Set #64 (circle 3)
Picture
Info
Domain Eukarya
Kingdom Protista
Uses contractile vacuole to regulate osmotic pressure
Cilia on the outside of cell to help with movement (these make it fuzzy looking on the edge)
The large red spot in the center is the nucleus
The clear-ish spot towards the left (by the pointy end) is the contractile vacuole
Slide #1 - Paramecium 100X) – Set #64 (circle 3)

3. Picture
Info
No domain/kingdom because “not living”
Flu mutates rapidly, and recovery from one form does NOT make you immune to another form, hence yearly flu shots
Polio doesn’t mutate rapidly, and we have a vaccine to prevent the only 3 forms of the virus out there
Other human diseases caused by viruses are Smallpox, Chicken Pox, Herpes, Measles, Mumps, Ebola hemorrhagic fever, and HIV
Slide #2 – Human Viruses (Left Side - 170,000x, & Right Side - 290,000X) – Set #97 (circle 3)

4. Picture
Info
Domain Eukarya
Kingdom Fungi
Contains molds, mildews, mushrooms, and yeasts in this kingdom
Heterotrophic (can’t make their own food)
Can be unicellular or multicellular
Many fungi have hyphae (finger-like projections that absorb nutrients), which create a mat called a mycelium
Sexual or asexual reproduction
Most of their life is spent in a haploid (as opposed to diploid) state
Slide #3 – Mushroom (Left Side – ½X, & Right Side – 400X) – Set 241 (circle 6)

5. Slide #4 – Gram-Negative Bacteria (200X) – Set 241 (circle 2)
Picture
Info
Domain Bacteria
Kingdom Eubacteria
Single-celled organisms that lack a nucleus (and membrane-bound organelle), have circular chromosomes, are smaller than Eukaryotic cells, and contain a peptidoglycan layer in their cell walls
These are gram-negative bacteria shown, which have a THIN peptidoglycan layer, and stain pink in the Gram Stain. Gram Positive bacteria would look purple after a Gram Stain, as they have a THICK peptidoglycan layer
Can be heterotrophs or autotrophs
Shapes of bacteria can be classified as bacillus (rod), coccus (sphere), or spirillium (helix) shaped – these are bacillus
Slide #4 – Gram-Negative Bacteria (200X) – Set 241 (circle 2)

6. Slide #5 – Gram-Positive Bacteria (2,000X) – Set #20 (circle 6)
Picture
Info
Domain Bacteria
Kingdom Eubacteria
Single-celled organisms that lack a nucleus (and membrane-bound organelle), have circular chromosomes, are smaller than Eukaryotic cells, and contain a peptidoglycan layer in their cell walls
These are gram-positive bacteria shown, which have a THICK peptidoglycan layer, and stain purple in the Gram Stain. Gram Negative bacteria would look pink after a Gram Stain, as they have a THIN peptidoglycan layer
Can be heterotrophs or autotrophs
The giant circle at the end of some of these rod-shaped (bacillus) bacteria are spores being formed
Slide #5 – Gram-Positive Bacteria (2,000X) – Set #20 (circle 6)

7. Slide #6 – Volvox (@ 150X) – Set #12 (circle 5)
Picture
Info
Domain Eukarya
Kingdom Protista
These are a multi-cellular, photosynthetic, plant-like Protist
The little spheres inside the big sphere are “daughter” volvox that will be released when the parent fills up with new daughters
Slide #6 – Volvox 150X) – Set #12 (circle 5)

8. Slide #7 – Algae (@ 175X) – Set #12 (circle 2)
Picture
Info
Domain Eukarya
Kingdom Protista
These are unicellular, photosynthetic, plant-like protists
Algae produce over 50% of the world’s oxygen
Many small animals feed on the algae
Algae are classified according to their color (green, red, and brown)
Slide #7 – Algae 175X) – Set #12 (circle 2)

9. Slide #8 – Amoeba proteus -Set #12 (circle 3)
Picture
Info
Domain Eukarya
Kingdom Protista
These are unicellular, non-photosynthetic, animal-like protists
Irregularly shaped, and can change shape constantly
It has pseudopods (false-feet) to help them with movement
Asexual reproduction, but can also conjugate
Slide #8 – Amoeba proteus -Set #12 (circle 3)

10. Slide #9 – Saccharomycete Yeast -Set #63 (circle 2)
Picture
Info
Domain Eukarya
Kingdom Fungi
Reproduces by budding
Unicellular
Heterotrophic
Can grow aerobically or anaerobically
When anaerobically grown, the yeast ferment sugars to make alcohol
Slide #9 – Saccharomycete Yeast -Set #63 (circle 2)

11. Slide #10 – Coccus Bacteria - Set #19 (circle 2)
Picture
Info
Domain Bacteria
Kingdom Eubacteria
Heterotrophic or Autotrophic
Unicellular
These are spherically-shaped (cocci) bacteria. They are found in clusters of 4 (called tetrads)
It can be difficult to see the shape without a higher power magnification, because bacteria are so much smaller than eukaryotic cells X magnification is the minimum for good resolution of the shapes of these bacteria (100X objective lens x 10X ocular lens)
Slide #10 – Coccus Bacteria - Set #19 (circle 2)

12. Slide #11 – White-Cap Mushroom (@ 1X) – Petri Dish Sample
Picture
Info
Domain Eukarya
Kingdom Fungi
Phylum Basidiomycota
Take note of the cap, stalk, ring (if present), volva (if present), and gills
The basidia line the gills – these are responsible for producing the spores for fungal reproduction
Slide #11 – White-Cap Mushroom 1X) – Petri Dish Sample

13. Slide #12 – Yeast (@ 1X) – Petri Dish Sample
Picture
Info
Domain Eukarya
Kingdom Fungi
Phylum Ascomycota
Reproduces by budding
Unicellular
Heterotrophic
Can grow aerobically or anaerobically
When anaerobically grown, the yeast ferment sugars to make alcohol
Slide #12 – Yeast 1X) – Petri Dish Sample