1. Experiment Questions
Enzyme pH

2. Enzyme pH
Name the enzyme and substrate that you used when you carried out an experiment to investigate the effect of pH on the rate of enzyme action. Enzyme + Substrate Catalase and H2O2 Amylase and Starch

3. Explain how you varied the pH? Used different Buffers
Enzyme pH
Explain how you varied the pH? Used different Buffers

4. Enzyme pH
State one factor that you kept constant. Temperature/enzyme conc./substrate conc.

5. The pH at which enzymes works best is known as the … pH. Optimum
Enzyme pH
The pH at which enzymes works best is known as the … pH. Optimum

6. Enzyme pH
Explain what is meant by the term pH. (A measure of) how acidic or alkaline a solution

7. Enzyme pH
For what purpose did you use buffer solutions in the course of your practical activities? To control the pH

8. Enzyme pH
Name (i) The enzyme you used in this investigation. (ii) The substrate of this enzyme. (iii) The product of this enzyme. (i) Amylase or Pepsin or Catalase (ii) Matching substrate (iii) Matching product

9. How did you vary the pH? Add acid or add base or add different buffers
Enzyme pH
How did you vary the pH? Add acid or add base or add different buffers

10. Enzyme pH
(i) Name one factor you kept constant. (ii) How did you keep that factor constant? (i) Temperature (ii) Water bath