1. Do Now Take out your: Vocabulary notes + Toothpickase lab
Answer the following questions:
What are the three factors that affect the rate at which an enzyme operates?
On the graph, which pathway is NOT catalyzed?
What letter corresponds to activation energy?
Write 2-3 sentences describing an analogy for an enzyme.

2. ENZYME INHIBITION

3. Inhibitors
Inhibitors are chemicals that reduce the rate of enzymic reactions
The are usually specific and they work at low concentrations
They block the enzyme but they do not usually destroy it
Many drugs and poisons are inhibitors of enzymes in the nervous system

4. The effect of enzyme inhibition
Irreversible inhibitors: Combine with the functional groups of the amino acids in the active site, irreversibly
Examples: nerve gases and certain pesticides

5. The effect of enzyme inhibition
Reversible inhibitors: Binds to the active site, but can be removed

6. The effect of enzyme inhibition
Competitive: These compete with the substrate molecules for the active site
The inhibitor’s action is proportional to its concentration
Resembles the substrate’s structure closely
Enzyme inhibitor complex
Reversible reaction
E + I EI

7. The effect of enzyme inhibition
Non-competitive: These are not influenced by the concentration of the substrate. It inhibits by binding irreversibly to the enzyme but not at the active site
Examples

8. Applications of inhibitors
Poisons snake bite, plant alkaloids and nerve gases
Medicine antibiotics, sulphonamides, sedatives and stimulants