1. Role of anti-Galα1,3Gal and anti–platelet antibodies in hyperacute rejection of pig lung by human blood 
Steffen Pfeiffer, MD, George L Zorn, MD, Sean Kelishadi, BS, Rafael Oriol, MD, Philippe Wolf, MD, Richard N Pierson, MD, Agnes M Azimzadeh, PhD 
The Annals of Thoracic Surgery 
Volume 72, Issue 5, Pages (November 2001)
DOI: /S (01)

2. Fig 1
Kaplan-Meier cumulative survival (%) of ex vivo perfused lungs. Survival of lungs from αGal and lung preperfusion groups are almost identical and therefore superimposed. (PPE = porcine platelet extract.)
The Annals of Thoracic Surgery  , DOI: ( /S (01) )

3. Fig 2
Anti–αGal antibody levels in arbitrary units (AU) over time. Different antibody class (IgM, IgG) levels measured in different treatment groups. Efficiency of the different treatment strategies for depletion of anti–αGal directed antibodies (IgM and IgG) (expressed as % remaining = measurable antibody). Values corrected for lung specificity by subtracting levels of antibody detectable at 30 minutes of lung perfusion experiment (and therefore not binding to lung tissue) from baseline values using the equation: % remaining antibody = Titer0min − Titer30 min/Titerpre absorption − Titer30 min × 100%. Figures of more detailed illustration of efficiency during single experiments are available directly from the authors. (PPE = porcine platelet extract.)
The Annals of Thoracic Surgery  , DOI: ( /S (01) )

4. Fig 3
Anti–PPE antibody levels in arbitrary units (AU) over time. Different antibody class (IgM, IgG) levels measured in different treatment groups. Efficiency of the different treatment strategies for depletion of anti–PPE directed antibodies (IgM and IgG) (expressed as % remaining = measurable antibody). Values corrected for lung specificity by subtracting levels of antibody detectable at 30 minutes after lung perfusion (and therefore not binding to lung tissue) from baseline values using the equation: % remaining antibody = Titer0 min − Titerpre absorption − Titer30 min × 100%. Figures of more detailed illustration of efficiency during single experiments are available directly from the authors. (PPE = porcine platelet extract.)
The Annals of Thoracic Surgery  , DOI: ( /S (01) )

5. Fig 4
Elaboration of complement C3a during the experiment expressed (in ng/mL) as levels at the respective timepoint − levels at the beginning of the experiment (t = 0 minutes).
The Annals of Thoracic Surgery  , DOI: ( /S (01) )

6. Fig 5
Elaboration of soluble complement C5b-9 during the experiment expressed (in ng/ml) as levels at the respective timepoint − levels at the beginning of lung perfusion (t = 0 minutes).
The Annals of Thoracic Surgery  , DOI: ( /S (01) )

7. Fig 6
Immunohistological analysis of pig lungs after 10 minutes of perfusion with human blood (staining for IgM in the left panels, for C3b in the right panels). Lungs used for antibody adsorption show bright staining for IgM and C3b. Lungs from the αGal group show week staining for IgM and IgG, suggesting that despite depleting all αGal-specific antibodies, there are still lung-specific antibodies binding to the graft during perfusion. Staining for C3b is negative, indicating that those bound antibodies do not activate complement. Lungs from lung perfusion group do stain negative for antibodies, suggesting that all lung-sensitive antibodies had been depleted.
The Annals of Thoracic Surgery  , DOI: ( /S (01) )