1. Microscope
Basics 1

2. Base
-Single piece of metal
-Support here when moving
→Color RED

3. Arm
-Hold here when moving
→Color Dark GREEN

4. -Hole allows light to pass through
Stage
-Supports Specimen
-Hole allows light to pass through
→ Color BLUE

5. -controls amount of light -ranges 1-5 →Color LIGHT GREEN
Diaphragm
-controls amount of light
-ranges 1-5
→Color LIGHT GREEN

6. Stage Clips
-secures specimen/slide
→ Color GREY

7. -NEVER use the sunlight it will hurt your eyes!!
Bulb/Mirror
-light source
-NEVER use the sunlight it will hurt your eyes!!
→Color YELLOW

8. Scanning Objective Lens
-shortest lens
-4x magnification
→Color BLACK

9. Low Power Objective Lens
-10x magnification
→Color PURPLE

10. High Power Objective Lens
-40x magnification
→Color ORANGE STRIPES

11. -allows light from objective to pass upward
Body Tube
-allows light from objective to pass upward
→Color BROWN

12. -Site of observation -10x magnification →Color ORANGE
Eye Piece/Ocular lens
-Site of observation
-10x magnification
→Color ORANGE

13. -Allows you to switch objective lenses
Nose Piece
-Allows you to switch objective lenses
→Color BLUE and GREEN stripes

14. Coarse Adjustment Knob
-used to focus specimen
-DO NOT use on high power objective lens
→Color PINK

15. -Used for minute focusing -Used after coarse adjustment knob
Fine Adjustment Knob
-Used for minute focusing
-Used after coarse adjustment knob
→Color Tan

16. Total magnification = (ocular lens power) x (objective lens power)
Example: (10x)oculear lens x (40x)high power lens = 400x total magnification
Magnification: The ability to increase the size of an image
Objective Lens
Ocular Lens X
= 400x total magnification

17. Light Pathway: Light Source→Specimen→Objective Lens→Body Tube→Eye Piece

18. ability to determine between two objects or points
Resolution
making the image clear
ability to determine between two objects or points
can be adjusted with the fine/course knob or the diaphragm (to allow more light in)

19. Comparing Powers of Magnification
We can see better details with higher the powers of magnification, but we cannot see as much of the image.
Which of these images would be viewed at a higher power of magnification? 19

20. Use of a Scope Carry the microscope with 2 hands (arm & base)
Use closest electrical outlet without going through a walkway
Looking at a slide:
Use ONLY ONE slide at a time
ALWAYS start at LOW power first(10x)
ALWAYS start with the stage lowered completely
Make sure the objective clicks into position
The course focus knob is ONLY used on the lowest power lens
The fine focus knob is used on all lenses to bring the image into focus 20

21. Putting the Microscope Away
Lower the stage completely and click the lowest objective into place
Return slide to tray
Wind up the scope power cord 21

22. Biological Drawings Drawing is an important skill to have in biology.
Draw what you see, not what you think you should see.
Avoid unnecessary detail.
Drawings will help you remember its features at a later date (ex: test)
Pay attention to detail and use PENCIL.
Color using color pencils.
Accurate title and labels should be included

23. Dicot Stem Cross Section Example

24. Copepod Example

25. Agenda Finish Microscope Handout Study Guide Extra Credit Flashcards
Glue in notebook when finished
Due tomorrow
Study Guide
Answer on separate piece of paper
Due Friday
Extra Credit Flashcards
Make 20

26. Types of Microscopes
1. Compound Light Microscope: Shines light through a specimen and uses 2 lenses for magnification
Magnification – increase in an objects apparent size
Resolution – how clear the image appears once magnified
Max clear magnification ~2000x
2. Electron microscope: Uses electrons instead of light to visualize the specimen, electrons bounce off or pass through and a computer interprets a picture
Always black and white, specimen must be dead to examine
Scanning EM – magnifies up to 100,000x
Transmission EM – magnifies up to 200,000x 26

27. Microscope Images Compound Light Microscope - Protozoa
Scanning Electron Microscope (SEM)
Butterfly Tongue
Transmission Electron Microscope - Bacteria 27