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Semen analysis: looking for an upgrade in class
Christopher De Jonge, Ph.D. Fertility and Sterility Volume 97, Issue 2, Pages (February 2012) DOI: /j.fertnstert Copyright © Terms and Conditions
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Figure 1 Automated semen analysis platform using lens-free on-chip microscopy (51). (A) The entire lens-free platform connected by a USB cable to a PC or smart phone for both power and control. (B) Schematic representation of the stage assembly and LED light source that provides a holographic image of the semen sample. Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © Terms and Conditions
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Figure 2 Sperm trajectories using lens-free on-chip microscopy (51). (A) Sperm trajectories with endpoints as blue spots and the trajectory path as a green line. (B) Speed distributions of 221 auto-tracked spermatozoa. Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © Terms and Conditions
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Figure 3 Microfluidic system for semen analysis (52). (Bottom, left) Sperm flow through a fluid-filled channel beneath two electrodes (electrodes are white horizontal images relative to the more grey vertical fluid-filled channel). (Bottom, right) Cells passing beneath an electrode cause a change in the electrical resistance. The resulting impedance “spike” signals are processed to allow differentiation between sperm cells and beads. Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © Terms and Conditions
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