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Detection and Quantification of BCR-ABL1 Fusion Transcripts by Droplet Digital PCR  Lawrence J. Jennings, David George, Juliann Czech, Min Yu, Loren Joseph 

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Presentation on theme: "Detection and Quantification of BCR-ABL1 Fusion Transcripts by Droplet Digital PCR  Lawrence J. Jennings, David George, Juliann Czech, Min Yu, Loren Joseph "— Presentation transcript:

1 Detection and Quantification of BCR-ABL1 Fusion Transcripts by Droplet Digital PCR 
Lawrence J. Jennings, David George, Juliann Czech, Min Yu, Loren Joseph  The Journal of Molecular Diagnostics  Volume 16, Issue 2, Pages (March 2014) DOI: /j.jmoldx Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2 Figure 1 Log-log plot demonstrating correlation of BCR-ABL1/BCR ratios to IS calibrators. Total RNA was extracted from pooled patient samples and diluted in total RNA from healthy donors. Replicates were tested together with IS calibrators by two technologists over 20 days. Data show near perfect correlation between sample dilutions and calibrators. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3 Figure 2 Log transformations of BCR-ABL1/BCR ratios show linearity and lack of bias. For both the dilution samples and the IS calibrator samples, the slope equals 1, indicating no concentration-dependent bias, and R2 is nearly 1, indicating remarkable correlation. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions


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