1. The amygdala: Site of genomic and nongenomic arousal of aldosterone-induced sodium intake 
Randall R. Sakai, Ph.D., Bruce S. Mcewen, Steve J. Fluharty, Li Yun Ma 
Kidney International 
Volume 57, Issue 4, Pages (April 2000)
DOI: /j x
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2. Figure 1
Daily 0.5 mol/L NaCl intake of animals treated with subcutaneously administered deoxycorticosterone (DOCA; 2.5 mg/day, N = 8 per group). Sodium intake aroused by DOCA treatment is suppressed by pulse intracerebroventricular (pICV) mineralocorticoid receptor (MR) antisense oligonucleotides (500 ng/day); the appetite returns to levels similar to that of vehicle-treated animals when the treatments are switched to pICV glucocorticoid receptor (GR) antisense oligonucleotides. The sodium intake of all animals returned to basal levels when all treatments were terminated.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

3. Figure 2
Effect of daily bilateral intraparenchymal injections of mineralocorticoid receptor (MR) or glucocorticoid receptor (GR) antisense oligonucleotides into the amygdala in established deoxycorticosterone (DOCA)-induced sodium appetite rats. The suppression of sodium intake was produced by MR antisense oligonucleotides treatment, but was reversed when scrambled/random oligonucleotides were administered. The treatment of a separate group of animals with GR antisense oligonucleotides was ineffective in suppressing DOCA-induced sodium intake. Symbols are: (○) pICV vehicle; (•) pICV MR or GR antisense. *P < 0.05 vs. saline + DOCA.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

4. Figure 3
Coronal section of rat brain, taken from the Paxinos and Watson Rat Brain Atlas, which shows the location of the cannula terminating in the amygdala. Only rats in which their implants terminated in this brain area were used in the analysis.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

5. Figure 4
Bilateral implants of steroid and their tetrahydro derivatives (TH-ALDO or TH-DOC) directly into the amygdala produced a rapid increases in 0.25 mol/L NaCl intake as compared with blank or cholesterol implants (*P < 0.05). Symbols are: (□) blank; (♦) cholesterol; () ALDO; (○) TH-ALDO; (▪) DOCA; (▵) TH-DOCA. Results are presented as mean ± SEM.
Kidney International  , DOI: ( /j x)
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6. Figure 5
An intake of 0.25 mol/L NaCl was increased by bilateral implants of (A) ALDO or TH-ALDO, and (B) DOC or TH-DOC. (A) ALDO or (B) DOC-induced 0.25 mol/L NaCl intake is inhibited by one-hour pretreatment with mineralocorticoid receptor antagonist RU (*P < 0.05 in A and B), but not by glucocorticoid receptor antagonist RU 486 (B). RU or RU 486 is not effective on 0.25 mol/L NaCl intake induced by TH-ALDO or TH-DOC (seen in A and B). Symbols in A are: (○) blank; (□) ALDO; (▪) TH-ALDO; (•) ALDO + RU 28318; (▲) TH-ALDO + RU Symbols in B are: (○) blank; (+) DOCA; (▪) TH-DOCA; (▴) DOCA + RU 28318; (▵) DOCA + RU 486; (□) TH-DOCA + RU 28318; (•) TH-DOCA + RU 486. The results are presented as mean ± SEM.
Kidney International  , DOI: ( /j x)
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7. Figure 6
An intake of 0.25 mol/L NaCl is increased by flunitrazepam and TH-DOC. Pretreatment with the GABA receptor antagonist Ro or Ro inhibited flunitrazepam and TH-DOC saline intake. Symbols are: (□) blank; (▪) flunitrazepam; (▵) TH-DOCA; (▲) ROl flunitrazepam; (○) ROl TH-DOCA; (•) ROl TH-DOCA.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions