1. Comparison between CLIA & ELISA
Inclination towards High throughput Advanced Technique
over Conventional Method of Diagnosis
Rajeev Kumar, N. Nanda Gopal, Richa Baranwal, Rajesh Sharma, Reba Chhabra, Surinder Singh
National Institute of Biologicals (NIB)
Ministry of Health & Family Welfare, Government of India, NOIDA
Abstract
Immunoassays are valuable tools for the detection of blood borne diseases and per se are widely used for detection of specific antigen and / or antibodies present in the sample of interest in all over world. In the past three decades so many new principle based immunoassays for screening of blood borne diseases like Hepatitis B Virus (HBV), Hepatitis C Virus (HCV) and Human Immunodeficiency Virus (HIV) comes in the Indian market viz Rapid, Enzyme Linked Immunosorbent Assay (ELISA), Enzyme Linked Fluorescent Assay (ELFA) and Chemiluminescence Immuno Assay (CLIA). Hence, quality of these kits needs to be tested before release in the market for safe guarding of our population.
Schematic Representation A. ELISA, B. CLIA
Comparison between CLIA & ELISA
CLIA
ELISA
High sensitivity
Measure Relative light unit(RLU)
Detection range is high
Short duration
Expensive
No stop solution
Comparatively low sensitivity
Measure optical density(OD value)
Detection range is low
Long duration
Low-priced
Use stop solution
Methodology
Conventional Technique
High throughput technique
ELISA
CLIA
CLIA-Chemiluminescence Light Immunoassay
Chemiluminescence is the production of light from a chemical reaction.
Two chemicals react to form an excited (high-energy) intermediate,
When the excited intermediates return back to their stable ground state, a photon is released, which is detected by the luminescent signal instrument.
A + B -> AB* -> Products + Light
Luminescent reactions are measured in Relative Light Units (RLU) that are proportionate to the amount of analyte present in a sample    
Inclination towards CLIA technology
Conclusion
Benefits of CLIA
CLIA is a better alternative method over conventional or traditional assays particularly ELISA for detection of antigen and / or antibodies of blood borne viruses.
It can help in detecting early infection compared to ELISA and is suitable in large sample volume laboratories.
Ultra-sensitive and can detect small amounts of the biological analyte.
CLIA has advantages of being more reliable, precise
technically simple and short incubation time.
CLIAs have improved specificity and better positive predictive value.
For Any other Information/Suggestions/Query, Kindly Contact:
Dr. Reba Chhabra, Deputy Director (Quality Control) i/c Diagnostics