1. Aim: How do instruments and techniques help science
Aim: How do instruments and techniques help science? Do Now: Does a writing utensil help you in school? How?

2. I. Compound light Microscope:
Used to study very small organisms.
Ex: Cells

3. B. Uses two lenses:
Ocular (Eye piece) lens
10x magnification
2. Objective lenses (nose piece)
Low Power: 10x magnification
High Power: 40x magnification

4. ocular (eyepiece)
Coarse Adjustment
Body tube
Fine Adjustment
Revolving Nosepiece
Arm
Lower Power Obj.
Stage Clips
High Power Obj.
Stage
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Diaphragm
Base
Light Source

5. Magnification Calculations
2. Magnification = power of objective lense X power of ocular lense
Ex.) High power objective lens X ocular lens = ?
__________ x __________ = ________
a. resolving power - ability to separate
two objects close together

6. Calculating Field of View (FOV)
3. Measurement
a. the Micrometer (um) 1 um = 1/1000 mm OR 1000 um = 1 mm
b. 1 um = .001 mm / 1 mm = um

7. Measurements Practice
miLlimeters to micRometers
 _______
_________ 
 ________
_______ 

8. FOV
How many millimeters
do you see? ______
how many um? _____

9. FOV
How many millimeters
do you see? ______
how many um? _____

10. Animations for practice
microscope parts
Virtual irchin

11. II. Staining
A way to make a microscopic (very small) image more visible under a microscope.
Ex: Iodine and Methylene Blue are stains used to make tiny cells easier to see.