1. Aerobic Respiration: Overview
Occurs in Four Distinct Stages:
Glycolysis: 10-step process in the cytoplasm.
Pyruvate Oxidation: 1-step process in the mitochondrial matrix.
Krebs Cycle: 8-step cyclical process in the mitochondrial matrix.
Electron Transport Chain & Chemiosmosis: Multi-step process in the inner mitochondrial membrane.
Krebs Cycle also known as the tricarboxylic acid cycle, TCA cycle or the citric acid cycle.
Chemiosmosis is also known as oxidative phosphorylation.

2. Energy Transfer Terminology
Substrate-level Phosphorylation:
ATP forms directly in an enzyme-catalyzed reaction.
Oxidative Phosphorylation:
ATP forms indirectly through a series of enzyme-catalyzed redox reactions involving oxygen as the final electron acceptor.
Need to cover this terminology before moving onto aerobic cellular respiration.
Substrate-level phosphorylation: a phosphate-containing compound transfers a phosphate group directly to ADP, forming ATP. For each glucose molecule processed, 4 ATP are produced this way in glycolysis and 2 in the Krebs cycle.

3. Energy Carriers
NAD+ and FAD+ are low energy, oxidized coenzymes that act as electron acceptors.
When an electron(s) are added to these molecules, they become reduced to NADH and FADH2.
In this case, reducing a molecule gives it more energy.

4. Cellular Respiration: The Details

5. Glycolysis
2 ATPs are used in steps 1 & 3 to prepare glucose for splitting.
F 1,6-BP splits into DHAP and G3P.
DHAP converts to G3P.
2 NADH are formed in step 6.
2 ATP are formed by substrate-level phosphorylation in both steps 7 and 10.
2 pyruvates are produced in step 10.
Glucose is phosphorylated in step 1 so that it becomes trapped in the cell and is more chemically reactive.
Glucose 6-phosphate is converted to its isomer, fructose 6-phosphate.
Glucose is phosphorylated again in step 3, so that there is one phosphate group on either end of the molecule…the sugar is now ready to split.

6. Glycolysis Energy Yield & Products:
4 ATP produced – 2 ATP used = 2 net ATP
2 NADH
2 pyruvates
Further processing in aerobic cellular respiration (if oxygen is available)
NADH is shuttled to the electron transport chain if there is oxygen available.

7. Folds of the inner membrane Fluid-filled intermembrane space
Mitochondria
Smooth
Highly folded
Folds of the inner membrane
Protein-rich liquid
Fluid-filled intermembrane space
Must go over the structure of the mitochondria before moving onto aerobic cellular respiration.

8. See PDF Glycolysis

9. Pyruvate Oxidation (if oxygen is present…)
The following occurs for each pyruvate:
CO2 removed.
NAD+ reduced to NADH and the carbon compound becomes acetic acid.
Coenzyme A (CoA) attaches to acetic acid to form acetyl-CoA.
The CO2 removed was the carboxyl group on the pyruvate.
When NAD is reduced it removes an H+ from the compound…
NADH is shuttled to the electron transport chain.
The fate of acetyl-CoA depends on the levels of ATP in the cell…
If ATP levels are low, acetyl-CoA goes into the Krebs cycle to increase ATP production.
If ATP levels are high, acetyl-CoA goes on to produce lipids…this is why we gain fat when we consume more food than we require.
All nutrients, whether protein, lipid or carbohydrate, are converted to acetyl-CoA and are then chanelled toward fat production or ATP production.

10. Pyruvate Oxidation

11. Pyruvate Oxidation Energy Yield & Products: 2 NADH 2 acetyl-CoA
2 CO2 (released as waste)
Per glucose

12. See PDF Pyruvate Oxidation

13. Occurs twice for each molecule of glucose, 1 for each acetyl-CoA.
The Krebs Cycle
Discovered by Hans Krebs in 1937
8-step cyclic process with oxaloacetate, the product of step 8, being the reactant in step 1
Occurs twice for each molecule of glucose, 1 for each acetyl-CoA.

14. The Krebs Cycle
In step 1, acetyl-CoA combines with oxaloacetate to form citrate.
In step 2, citrate is rearranged to isocitrate.
NAD+ is reduced to NADH in steps 3, 4 and 8.
FAD is reduced to FADH2 in step 6.
ATP if formed in step 5 by substrate-level phosphorylation. The phosphate group from succinyl-CoA is transferred to GDP, forming GTP, which then forms ATP.
In step 8, oxaloacetate is formed from malate, which is used as a reactant in step 1.
CO2 is released in steps 3 and 4.
After step 1, CoA released and is available to process another pyruvate molecule.
GDP stands for guanosine diphosphate

15. The Krebs Cycle Energy Yield & Products: 2 ATP 6 NADH 2 FADH2
4 CO2 (released as waste)
NADH and FADH2 carry electrons to the electron transport chain for further production of ATP by oxidative phosphorylation.

16. See PDF Krebs