1. Students
Get handout – Enzyme Lab (if absent yesterday)
Food & drink…put aside on back tables…..thank you
Today is Pearl Harbor Day
Cell phones in bin….muted or off….please & thank you

2. Lab 2: Enzyme catalysis
Guaiacol + 2H2O tetraguaiacol H2O
(colorless) (brownish orange)
Enzyme = peroxidase
Substrate = peroxide (H2O2)
How will we measure enzyme function?
- Color change using a spectrophotometer
peroxidase

3. How does a spectrophotometer work?
Take home points
Light at a specific wavelength is sent through a liquid sample
Light passing through the sample is detected on the other side
To measure this accurately, we must calibrate (blank) the spec.
Calibrating the spec sets the range & “tells” the spec:
what 0% light is (darkness)
what 100% light is (all light passing through….the blank tube)
For our experiment we will see how the amount of light passing through the test tube changes as the reaction proceeds.

4. Tube #1
(blank)
Tube #2
Tube #3
Guaiacol
0.1 mL
(2 drops)
---
H2O2
0.2 mL
(4 drops)
Turnip extract
(peroxidase)
1.0 mL
(20 drops)
dH2O
5.2 mL
5.0 mL
Set wavelength to 500 nm
With the chamber empty, use left knob to adjust to 0% T
Insert tube #1 (blank) into spec & use right knob to adjust to 100% T
Pour #2 into #3 & begin timing immediately!!
Invert 2X quickly & place into spec
Record %T every 10 sec for 90 – 120 secs (do 2X with 2 new tubes)
From page 3, choose your experiment for tomorrow
Peel tape, rinse tubes & place in tan basin by sink. Thank you!
Graph data together on 1 graph & calculate reaction rates of each line

5. Students
Ladies – Perry WFU
Hands-on workshops, orthopedic surgeons, biomechanics
Today is National Brownie Day
Pretend to be a Time Traveler Day
Food & drink away or on the back table
Phones in the bin….muted or off…please & thank you!
Experimental designs….MUSTS!!!!
You MUST re-do yesterday’s experiment
Different enzyme prep = different enzyme concentration
Divide roles to efficiently use your time!!

6. Students
Get handout – “The Biochemistry & …..”
Lab notebooks – MONDAY – Osmosis/diffusion & Enzyme
Today is National Pastry Day
Cell phones in bin….muted or off….please & thank you

7. Students
Osmosis/diffusion
Pre-lab can be same for both
Purpose/overview
Personal Acct
Diffusion
Procedure – paraphrased
Data table
Osmosis
Procedure
Your data & class data
Graph
Discussion questions for both
Conclusion for both

8. Enzyme Lab
Pre-lab
Enzyme info
Spec info
The Reaction we measured
Purpose/overview
Personal Acct
Day 1
Procedure with ingredient table
Data table – get data if you were absent
Graph & rxn rate
Day 2
Elements of Good Experimental Design
Data table
Graph & reaction rates
Discussion question…Did your data support or refute your hypothesis?
Conclusion
What did you learn?
What was the lab’s significance?
What is your revised hypothesis (if needed)?