1. Three-Dimensional Morphology of Touch Domes in Human Hairy Skin by Correlative Light and Scanning Electron Microscopy 
Mari Orime, Tatsuo Ushiki, Daisuke Koga, Masaaki Ito 
Journal of Investigative Dermatology 
Volume 133, Issue 8, Pages (August 2013)
DOI: /jid
Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Light micrographs of a human touch dome. (a) Hematoxylin–eosin-stained section of touch domes (small arrows) bordered by peripheral thick epidermal ridges (arrowheads). *Hair follicle. (b) Closer view of the region framed in a. Arrows: clear cells. (c) Immunohistochemical study for keratin 20 (K20) (brown) and protein gene product 9.5 (PGP 9.5) (violet). K20-immunopositive Merkel cells are in contact with PGP 9.5-positive nerve terminals (arrows). (d) Samples of K20-immunostained serial sections used for three-dimensional (3D) reconstruction. (e) 3D reconstruction from 44 serial sections. K20-immunopositive Merkel cells (pale red) are identified on the epidermal base of the touch dome. Note a circumferential epidermal ridge (arrowheads). Bars: a, d=100μm; b, c=10μm; and e=500μm.
Journal of Investigative Dermatology  , DOI: ( /jid )
Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Human touch domes viewed from the dermal side. (a, b) Whole-mount epidermal specimen immunostained for keratin 20, and observed by light microscopy and scanning electron microscopy (SEM). (c, d) Closer views of the framed areas in a and b. Portions of Merkel cells (numbered 1–11) appear covered with neuronal components because of their continuity with unmyelinated fibers (white arrowheads). (e, f) Touch domes (colored purple) observed by SEM. Note epidermal ridges (white arrows) dividing the dome into subcompartments. Thick arrow: duct of sweat gland. *Hair follicles. (g, h) Nerve fibers (white arrowheads) connect the neighboring Merkel cell–neurite complexes (white asterisks). Note nerve terminals (arrows in g) ending in the epidermis. S: Schwann cell body. Bars: a, b=100μm; c, d=10μm; e, f=100μm; and g, h=10μm.
Journal of Investigative Dermatology  , DOI: ( /jid )
Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions