1. Biology Do Now
Directions: Take out a sheet of notebook (Do Now sheet) and, then answer each question based on your notes.
What type of molecule is an enzyme?
What is the building block of an enzyme?
Most enzymes end in what suffix?
Why are enzymes called “biological catalysts”?

2. Biology Do Now
Directions: Take out a sheet of notebook (Do Now sheet) and, then answer each question based on your notes.
What type of molecule is an enzyme? protein
What is the building block of an enzyme? Amino acids
Most enzymes end in what suffix? -ase
Why are enzymes called “biological catalysts”? Enzymes are biological catalysts because they are found in living things and they speed up the rate of reactions.

3. Reminders
The unit 1 assessment will be rescheduled until next Wednesday, February 27, 2019.
The window for 3rd quarter progress report grades opens yesterday, 2/21/19 and closes on Friday, March 1, 2019 at 5 pm.
Check your average on Infinite Campus for each course.
Teachers have the right to close their 3rd quarter progress report grades as soon as TODAY, if they choose to.

4. Objective:
Students will know the factors that affect enzyme activity by performing 5 labs.
Mastery: 75% of better

5. Students will watch the video “HYDROGEN PEROXIDE AND CATALASE REACTION” Source: ENZYMES: “Helper” Protein molecules

6. The Effect of pH, Salt and Temperature on Catalase Activity
Unit I: Structure & Function
Friday, February 22, 2019

7. The factors we will investigate today are:
Salt pH
Acid (vinegar)
Base (baking soda)
Temperature
Hot (boiled potato)
Cold (refrigerated potato)

8. What will we measure? Amount of bubbles
++++ means lots of bubbles; foamy
+++ means many bubbles
++ means some bubbles
+ means a few bubbles
0 means no bubbles

9. Data table LAB EXPERIMENTS NUMBER OF BUBBLES Control Group ++++
 ++++
The effect of VINEGAR (acid) on catalase activity
The effect of BAKING SODA (base) on catalase activity
The effect of COLD temperature on catalase activity
The effect of HOT temperature on catalase activity
The effect of SALT on catalase activity

10. Hypotheses your lab group will test:
Hypothesis #1 (The effect of an acid on Catalase activity): If Catalase activity depends on the presence of an acid then, vinegar will increase Catalase activity, producing lots of bubbles.
Hypothesis #2 (The effect of a base on Catalase activity): If Catalase activity depends on the presence of a base then, baking soda will reduce Catalase activity, producing no bubbles.
Hypothesis #3 (The effect of a high temperature on Catalase activity): If Catalase activity depends on temperature then, a high temperature will reduce Catalase activity, producing no bubbles.
Hypothesis #4 (The effect of a low temperature on Catalase activity): If Catalase activity depends on temperature then, a low temperature will increase Catalase activity, producing lots of bubbles.
Hypothesis #5 (The effect of salt on Catalase activity): If Catalase activity depends on the presence of salt then, salt will increase Catalase activity, producing lots of bubbles.

11. Was hypothesis #1 correct?
Hypothesis #1: If Catalase activity depends on the presence of an acid then, vinegar will increase Catalase activity, producing lots of bubbles.
Procedure:
1) Find the flask labeled “V” for vinegar.
2) Add 2 potato cubes to the vinegar.
3) Let the potatoes sit in the vinegar for 1 minute.
4) Add 20 mL of hydrogen peroxide to the flask with the potatoes.
5) Observe the amount of bubbles and record your results in your data table.
Was hypothesis #1 correct?

12. 1) Find the flask labeled “B Soda” for baking soda.
Hypothesis #2: If Catalase activity depends on the presence of a base then, baking soda will reduce Catalase activity, producing no bubbles.
Procedure:
1) Find the flask labeled “B Soda” for baking soda.
2) Add 2 potato cubes to the baking soda solution.
3) Let the potatoes sit in the baking soda solution for 1 minute.
4) Add 20 mL of hydrogen peroxide to the flask with the potatoes.
5) Observe the amount of bubbles and record your results in your data table.
Was hypothesis #2 correct?

13. Was hypothesis #3 correct?
Hypothesis #3: If Catalase activity depends on temperature then, a high temperature will reduce Catalase activity, producing no bubbles.
Procedure:
1) Find the flask labeled “hot” for boiled potatoes
2) Add 2 boiled potato cubes to the flask labeled “hot”.
3) Add 20 mL of hydrogen peroxide to the flask with the boiled potatoes.
5) Observe the amount of bubbles and record your results in your data table.
Was hypothesis #3 correct?

14. Was hypothesis #4 correct?
Hypothesis #4: If Catalase activity depends on temperature then, a low temperature will increase Catalase activity, producing lots of bubbles.
Procedure:
1) Find the flask labeled “cold” for refrigerated potatoes
2) Add 2 boiled potato cubes to the flask labeled “cold”.
3) Add 20 mL of hydrogen peroxide to the flask with the boiled potatoes.
5) Observe the amount of bubbles and record your results in your data table.
Was hypothesis #4 correct?

15. Procedure: 1) Find the flask labeled “S” for salt.
Hypothesis #5: If Catalase activity depends on the presence of salt then, salt will increase Catalase activity, producing lots of bubbles.
Procedure:
1) Find the flask labeled “S” for salt.
2) Add 2 potato cubes to the salt solution.
3) Let the potatoes sit in the salt solution for 1 minute.
4) Add 20 mL of hydrogen peroxide to the flask with the potatoes.
5) Observe the amount of bubbles and record your results in your data table.
Was hypothesis #5 correct?

16. In your groups, write a conclusion that includes the following:
Explain how each factor affected catalase activity.
Use your SPECIFIC DATA from your data table.
Which factor(s) did catalase produce the MOST bubbles? Why?
Which factor(s) did catalase produce the LEAST bubbles? Why?