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Rapid Allelic Discrimination by TaqMan PCR for the Detection of the Gilbert's Syndrome Marker UGT1A1*28  Ursula Ehmer, Tim O. Lankisch, Thomas J. Erichsen,

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Presentation on theme: "Rapid Allelic Discrimination by TaqMan PCR for the Detection of the Gilbert's Syndrome Marker UGT1A1*28  Ursula Ehmer, Tim O. Lankisch, Thomas J. Erichsen,"— Presentation transcript:

1 Rapid Allelic Discrimination by TaqMan PCR for the Detection of the Gilbert's Syndrome Marker UGT1A1*28  Ursula Ehmer, Tim O. Lankisch, Thomas J. Erichsen, Sandra Kalthoff, Nicole Freiberg, Michael Wehmeier, Michael P. Manns, Christian P. Strassburg  The Journal of Molecular Diagnostics  Volume 10, Issue 6, Pages (November 2008) DOI: /jmoldx Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Typical example of the allelic discrimination of the UGT1A1*28 variant by TaqMan PCR. NTC, no template control. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 Analysis of 10 samples each of one wild-type, one heterozygous, and one homozygous probe shows low interprobe variability. Mean values for VIC and FAM fluorescence are shown for each probe with bars representing mean SD. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

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