1. Reduced Hyaluronan in Keloid Tissue and Cultured Keloid Fibroblasts
Ludger J.M. Meyer, Barbara M. Egbert, Svetlana Shuster, Robert Stern 
Journal of Investigative Dermatology 
Volume 114, Issue 5, Pages (May 2000)
DOI: /j x
Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Accumulation of HA in culture media and on cell layers of normal and keloid-derived fibroblasts. Cultures were grown to confluence under standard culture conditions. HA accumulated in the media during the last 48 h and on the cell layers of keloid strains 33 (solid black bar) and 125 (hatched black bar) and normal strains 21 (solid white bar) and 131 (hatched white bar) were measured as described in Materials and Methods and in the legend of Table 2.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Time course of HA accumulation in intact and ‘‘wounded’' monolayers of normal and keloid-derived fibroblasts. Four replicate T75 flasks of a normal and a keloid strain were grown to 80% confluency in standard culture medium. Two flasks of each cell type were then scratched as described in Materials and Methods and two were left undisturbed. (a) Represents normal scar fibroblast cultures, and (b), the results from keloid-derived fibroblast cultures. All flasks were incubated in fresh media either with serum (open squares and filled diamonds) or without serum (filled squares and open diamonds). HA accumulation was assayed at the indicated times. The uppermost lines in (a) and (b) (filled diamonds) represent accumulation in scratched cultures incubated in serum-containing medium. The second uppermost lines in (a) and (b) (open squares) represent accumulation of HA in intact (nonscratched) cell cultures. In each case, fibroblasts responded to the artificial wounding or scratch phenomenon with increased HA accumulation. The two lowest lines in (a) and (b) represent cells grown in the absence of serum and show very little HA accumulation. The open diamonds represent accumulation in scratched cultures in serum-free medium, whereas the filled squares represent intact nonscratched cultures.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
Size distribution of HA chains in the culture media of fibroblasts from normal scar and keloid. (a) Normal scar; (b) keloid. Following molecular sieve chromatography as described in Materials and Methods, HA determinations were performed on aliquots of each fraction. Fractions 12–14 represent the excluded volume (Vo), and contain high molecular weight HA polymers. In each case, in both keloid and normal fibroblast cultures, the HA that accumulates is found in the excluded or void volume.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

5. Figure 4
Tissue distribution of HA in dermis and epidermis of normal scar and keloid. HA in formalin-fixed, paraffin-embedded samples of normal human scar (A, D) and keloid (B, E) was stained using biotinylated HA-binding peptide as described in Materials and Methods. As a control (C) the HA-binding peptide was preincubated with HA before application to the slide. Staining in the epidermis is observed at higher magnification in normal scar (D) and keloid (E). Scale bars: (A–C) 200 mm; (D and E) 100 mm.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions