1. Volatile anaesthetic effects on phospholipid binding to synaptotagmin 1, a presynaptic Ca2+ sensor 
D. Fu, P. Vissavajjhala, Hemmings H.C.  
British Journal of Anaesthesia 
Volume 95, Issue 2, Pages (August 2005)
DOI: /bja/aei163
Copyright © 2005 British Journal of Anaesthesia Terms and Conditions

2. Fig 1
Role of synaptotagmin C2 domains in mediating Ca2+-dependent synaptic vesicle–membrane interactions. Interaction of Ca2+ with the tandem C2A and C2B domains of synaptotagmin 1 facilitates its interaction with negatively charged phospholipids in the plasma membrane. This interaction can trigger synchronous vesicle fusion mediated by further interactions with the t-SNARE proteins syntaxin and SNAP-25, components of the core vesicle fusion complex.
British Journal of Anaesthesia  , DOI: ( /bja/aei163)
Copyright © 2005 British Journal of Anaesthesia Terms and Conditions

3. Fig 2
Anaesthetic effects on phospholipid binding to C2 domains of synaptotagmin 1. Recombinant rat GST-synaptotagmin 1 fusion proteins containing the first (C2A), second (C2B) or both (C2AB) C2 domains were immobilized on glutathione–Sepharose beads. Beads were incubated with [3H]phospholipid liposomes composed of: (a) phosphatidylserine and phosphatidylcholine (PS/PC); or (b) phosphatidylinositol and phosphatidylcholine (PI/PC); in the absence or presence of Ca mM, halothane 1.26 mM, and/or isoflurane 1.04 mM, as indicated. Beads were washed and the bound phospholipids were quantified by liquid scintillation counting. Data are shown for a single representative experiment (n=3) as mean (sd) of triplicate determinations. There was no significant effect by either anaesthetic on binding (anova).
British Journal of Anaesthesia  , DOI: ( /bja/aei163)
Copyright © 2005 British Journal of Anaesthesia Terms and Conditions

4. Fig 3
Calcium concentration-dependence of PS/PC binding to C2A domain of synaptotagmin 1 with and without (a) halothane and (b) isoflurane. Recombinant rat GST-synaptotagmin 1 C2A domain fusion protein immobilized on glutathione–Sepharose beads was incubated with [3H]-labelled PS/PC liposomes in the presence of the concentrations of Ca2+ indicated in the absence or presence of halothane (a) or isoflurane (b). Data are shown for a single representative experiment (n=3) as mean (sd) of triplicate determinations. Data were fitted to sigmoidal concentration–effect curves for determination of Emax and EC50 values, which did not differ between experimental groups (anova).
British Journal of Anaesthesia  , DOI: ( /bja/aei163)
Copyright © 2005 British Journal of Anaesthesia Terms and Conditions