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Fig. 5 DMN-Tre labeling is selective for live mycobacteria.

Published byÖzgür Melek Yerlikaya Modified over 5 years ago

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Presentation on theme: "Fig. 5 DMN-Tre labeling is selective for live mycobacteria."— Presentation transcript:

1 Fig. 5 DMN-Tre labeling is selective for live mycobacteria.
DMN-Tre labeling is selective for live mycobacteria. (A and B) No-wash imaging (A) and flow cytometry MFI analysis (B) of live and heat-killed (95°C for 30 min) mCherry Msmeg cells in the presence of 100 μM DMN-Tre for 30 min. (C) Flow cytometry MFI analysis of Msmeg cells in exponential (log) or stationary growth phase incubated with 100 μM DMN-Tre for 1 hour or overnight (~16 hours). (D) Flow cytometry analysis of live or heat-killed cells resuspended with or without culture filtrates from live cells, followed by incubation with DMN-Tre as in (A). Images were collected in the DIC, FITC/GFP (for DMN fluorescence), or RFP (for mCherry fluorescence) channels of a Nikon A1R confocal microscope. Scale bars, 5 μm. In (B) and (C), data are means ± SEM from at least two independent experiments. Data were analyzed by two-way ANOVA test (****P < ). Mireille Kamariza et al., Sci Transl Med 2018;10:eaam6310 Published by AAAS

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