1. Restriction Digest gel template: Fill in the #s and Xs with the appropriate information.
##RDG##.10
Student Names
#XX#.10
#XX#.10
#XX#.10
#XX#.10
#XX#.10
#XX#.10 M U C U C U C U C U C U C
4000
Paste/Import cropped gel here and resize to fit markers (Note: Some markers and lanes may need to be moved to fit the gel).
Label lanes with the proper clone names and add student names.
Indicate the calculated size of the insert by RD and by PCR (from the PCRG on the samples)
Save file as the proper RDG name I.e. 20RDG1.10
Upload file to your school’s Drop box on Sakai
3000
2000
1650
1000
850
650
500
400
300
200
100
Insert size by RD
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Insert size by PCR
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