1. Daphnia Inquiry Lab
Ms. Kluesner
AP Biology

2. What are Daphnia? Tiny crustacean water fleas Ectotherms???
Arthropods- segmented animal with exoskeleton
Recognized by their jerky movement through water
Self replicating
What does it mean if there are differences between parent and offspring?
When food is scared eggs may need to be fertilized
Ectotherms- COLD BLOODED, body temperature changes with the environments
Arthropods- segmented animal with exoskeleton
* insects, spiders, millipedes, and crabs

3. Daphnia Anatomy Kidney-shaped
Leaf like limbs inside the carpace (clear outside skeleton)
Two antennae
The HEART is on the back
Leaf like limbs inside the carpace (clear outer skeleton) that produce a current of water that carries food and oxygen to the mouth and gills
Heart is on the back like other arthropods

4. Daphnia Heartbeat Video

5. Can you think of anything that could effect Daphnia heart rate?

6. Your Job!!
Propose a question and hypothesis about different aspects that could effect the heart rate of Daphnia
After completing the lab you will create a poster presentation of your self-designed lab
See rubric for further detail

7. Materials Daphnia Petri Dishes Dissecting scopes Pipettes
THINGS TO REMEMBER!
One drop of liquid
Do not keep them under the microscope for too long
Variety of chemicals
Caffeine
Ethyl alcohol
Nicotine
Adrenaline
Lactic Acid
Ringer’s Solution (Saline)
Aspirin Solution

8. GOOD LUCK!
Remember to tell them only one drop of liquid so the daphnia does not swim out of there view
Take heartbeat for ten seconds and then multiply by six and take measurements quickly so the daphnia do not become stressed in the small volume of water