1. The effect of ETC duration and temperature on imaging depth.
The effect of ETC duration and temperature on imaging depth. A 2mm thick coronal section before (A) and after (B) clearing with out optimized protocol. C, Coronal mouse brain section (2 mm thick) cleared with ETC for 1 d, 3 d, or 5 d at 37°C or for 4 d at 37°C plus an additional day at 55°C. Sections were subsequently stained with propidium iodide and imaged using a confocal microscope under identical excitation conditions without the use of Z-attenuation correction. There is an increase in depth at which a fluorescent signal may be imaged with respect to clearing protocol. The 37/55°C clearing temperature provides deepest imaging penetration.
Jonathan R. Epp et al. eneuro 2015;2:ENEURO
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