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Granulosa–lutein cell growth differentiation factor-9 (GDF-9) messenger RNA and protein expression in in vitro fertilization (IVF) cycles: relation to.

Published byἨώς Σπηλιωτόπουλος Modified over 5 years ago

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Presentation on theme: "Granulosa–lutein cell growth differentiation factor-9 (GDF-9) messenger RNA and protein expression in in vitro fertilization (IVF) cycles: relation to."— Presentation transcript:

1 Granulosa–lutein cell growth differentiation factor-9 (GDF-9) messenger RNA and protein expression in in vitro fertilization (IVF) cycles: relation to characteristics of ovulation induction and IVF  Hong-Yuan Huang, M.D., Hsin-Shih Wang, M.D., Ph.D., She-Hung Chan, B.S., Chyi-Long Lee, M.D., Chia-Woei Wang, M.D., Yung-Kuei Soong, M.D., M.Phil.  Fertility and Sterility  Volume 91, Issue 4, Pages (April 2009) DOI: /j.fertnstert Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

2 Figure 1 (A) Quantitative competitive PCR analysis of granulosa–lutein cell GDF-9 expression. (B) Summary of GDF-9 oligonucleotide primer sequences. (C) No additional statistically significant correlation demonstrated between GDF-9 mRNA levels and the serum estradiol level on hCG day (P=.211) or the number of retrieved oocytes (P=.575). (D) A statistically significant correlation demonstrated between GDF-9 mRNA concentration and the number of dominant follicles (>16 mm) on hCG day (P=.044), but none with the number of follicles <16 mm (P=.867). (E) Western blot analysis of follicular fluid GDF-9. A major band at 51 kd, representing GDF-9, was detected in individual follicular fluid samples (lanes 1–6). MW, molecular weight; P, recombinant GDF-9. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

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