1. Inspiring Advances in Bioprocessing

3. Inspiring advances in bioprocessing since 1985
AN INDUSTRY LEADER IN
Hollow Fiber and Flat Sheet Filtration Technologies
Pre-packed Chromatography Columns
Protein A Affinity Ligands Manufacturing
Most of the world’s monoclonal antibodies are purified on Repligen’s Protein A
Upstream and Downstream Applications
Chromatography
25-30%
Proteins
~40%
Filtration
30-35%
Repligen Corporation: one of the fastest-growing companies in
North America
Multiple global manufacturing sites
20 of the Top 25 Pharma companies use Repligen products

4. Rapid growth through innovative launches and strategic acquisitions
1985
1988
2010
2011
2012
2014
2015
2016
2017
Protein A ligands
commercial manufacturing
Launched Immobilized Protein A resins
Acquired
BioFlash technology:
Pre-packed columns
Acquired Novozymes Biopharma: Native Protein A, growth factors
Launched OPUS® pre-packed columns
Acquired Refine ATF technology: XCell™ ATF
Launched OPUS® 60
Acquired Atoll: OPUS™ PD
Acquired Spectrum® HF TFF Systems
Repligen is one of the fastest growing companies in
North America
Launched XCell™ Single-use, OPUS® R
Launched XCell™ 10 Single-use
Launched OPUS® 45
Acquired TangenX™ TFF Cassettes

5. Repligen products in biologics
process development

6. Repligen products in biologics commercial manufacturing
mAb and Vaccine based Production

7. Repligen products in continuous processing

8. Setting new standards in bioprocessing
XCell™ ATF Systems
Award-winning perfusion cell separation device
Stainless steel and single-use formats
Enables continuous processing
In 50+ Phase 1-3 processes and 4 commercial processes
Spectrum Filters and Systems
KrosFlo® Hollow Fiber Filters and Perfusion Systems
ProConnex™ modules, bags and tubing sets
Used in biologics development & manufacturing, gene therapy, vaccine production
TangenX™ TFF Filters
First purpose-built single-use TFF cassettes
Single-use and reusable formats
80% cost reduction, 50% labor reduction, 75% less buffer use
In 200+ Phase 1-3 processes and 5 commercial processes
LONG® Cell Culture Supplements
Up to 200 times more potent than insulin
Manufactured to ISO standards in Sweden
In 6+ commercial processes
OPUS® Pre-packed Columns
Leader of the pack in resin choice and size
From process development to large-scale commercial applications
In 100+ Phase 1-3 processes, 1 commercial process
Experts in column packing
ELISA Quantitation Kits
Fast, reliable, and re-producible quantitation of leached Protein A, MabSelect SuRe™ ligand and LONG®R³ IGF-I
Less text, better way to get pictures in
Optimizing process efficiency, process economics, and risk mitigation

9. Scaling to meet customer demands
Waltham, MA Shrewsbury, MA
Lund, Sweden
Ravensburg, Germany
Los Angeles, CA
Las Vegas, NV
Houston, TX Dallas, TX
Breda,
Netherlands
Growing global
presence,
Scaling to meet customer demands
As of October 2017
Direct Sales (34)
Field Applications Specialists (3)
Field Service Specialists (7)
Key Distributors (5)

10. Focus on business continuity, quality systems, and risk mitigation
Waltham, MA - Headquarters
Lund, Sweden
Ravensburg, Germany
Shrewsbury, MA
Los Angeles, CA
Las Vegas, NV
Dallas, TX
Houston, TX
Breda, NL, EU
Multi-Site Manufacturing
Safety Stock Program
Stringent Inventory Management Program
Animal-free
Robust supply chain
Secure Raw Material Supply Chains
Supports customer filings
Technical support
Complete Regulatory Support Packages
Global technical support
Field applications team
Hands-On Service and Support
Focus on business continuity, quality systems, and risk mitigation

11. Redefining modern bioprocessing: 2017 and
beyond
Single-use Systems
for flexible bioprocessing
Global
expansion and outreach
Continuous Processing
tools and systems
Europe manufacturing and center of excellence

12. Pre-packed Chromatography Columns
OPUS®
Pre-packed Chromatography Columns
Pre-packed chromatography columns with unparalleled flexibility in resin choice and size
Experts in packing manufacturing scale columns
Winner of multiple awards for design excellence
First production- scale pre-packed columns
In 100+ Phase 1-3 processes and 1 commercial process since its launch in 2012
Open platform,
user-specified

13. OPUS® PD Pre-packed Columns
Designed for process development and process validation, including viral clearance, scale-up, and sample preparation
Over resins packed
OPUS® RoboColumn® for automated parallel chromatography
OPUS® MiniChrom for process development and sample preparation
OPUS® ValiChrom for process validation
Pre-packed columns for process development (formerly Atoll Columns)

14. XCell™ ATF Systems
Award-winning perfusion cell separation device for high cell concentration and process intensification
Leading cell separation device in perfusion mAb and rProtein production
Single-use and stainless steel formats
Applications beyond perfusion; cell banking, seed train optimization
In 50+ Phase 1–3 processes and 4 commercial processes
Add in ATF products
For high density cell culture and process intensification

15. TangenX™ TFF Cassettes
Featuring the first purpose-built single-use cassettes for tangential flow filtration
Reliable, consistent performance since 2007
80% reduction in filter cost
50% reduction in labor use
75% reduction in buffer use
Interchangeable, single-use, and reusable formats
Scalable from bench to production
Reliable, scalable, cost-effective solutions for tangential flow filtration

16. Spectrum® KrosFlo hollow fiber filters for tangential flow filtration
KrosFlo TFF Hollow Fiber Filters
Scale-up made easy:
Spectrum® KrosFlo hollow fiber filters for tangential flow filtration
Scalable, from process development to commercial manufacturing
Fast processing, with high flux rates
High yield, low protein binding
Manufactured in compliance with ISO guidelines
Used in over Spectrum® Krosflo TFF Systems and over 2000 XCell™ ATF Systems worldwide
Compatible with other industry hollow fiber TFF systems
Pore size range: 1kD – 0.65µm
Filter module
Surface area
Volume
MicroKros
13 cm2 – 60 cm2
1-100 ml
MidiKros
75 cm2 – 370 cm2
100 ml - 3 L
MidiKros TC
MiniKros Sampler
490 cm2 – 2600 cm2
3 L -15 L
MiniKros
1550 cm2 – 8500 cm2
5 L - 50 L
KrosFlo
7850 cm2 – 4.1 m2
10 – 100L
KrosFlo MAX
4.3 m2 – 12.8 m2
100L – 1000+L
KrosFlo MAX
mPES (Modified Polyethersulfone) hollow fiber filters

17. Turnkey hollow fiber systems for tangential flow filtration
Spectrum® Standard KrosFlo TFF Systems
Turnkey hollow fiber systems for tangential flow filtration
Scalable from process development to commercial manufacturing
Volumes from milliliters to thousands of liters
Automated process control
Proprietary data collection software
Worldwide adoption, with over systems in use today
Custom systems available, including KTF, KrosFlo Pilot Plus, and KPS Systems
KMPi TFF System: L
KR2i TFF System: 1ml to 10L
KML 100 System: L

18. Protein A resins from the leading manufacturer of rProtein A ligands
CaptivA®
Protein A Resin
Protein A resins from the leading manufacturer of rProtein A ligands
Industry-standard results at a fraction of the cost of traditional resins
In 3 commercial processes, expanding pipeline of Phase 1–3 processes
The value choice in Protein A resins

19. LONG®R³ IGF-I Cell Culture Supplement
Cell culture supplement 200 times more potent and two times more stable in cell culture than insulin
Recombinant, animal origin-free, cGMP compliant
Used extensively in mAb and rProtein production
Secure global supply
In 6 commercial processes
Increasing adoption in biosimilar development, cell therapy, and vaccine applications
Potent, safe, flexible cell culture growth factor

20. ELISA Quantitation Kits
Quantitation of leached Protein A, MabSelect SuRe™ ligand, and LONG®R³ IGF-I
Most accurate matched standard for MabSelect SuRe™ resin
High specificity and high sensitivity detection and quantitation
Reproducible and consistent inter- and intra-kit consistency
Fast, reliable, reproducible analytics

21. Pre-packed Chromatography Columns
OPUS®
Pre-packed Chromatography Columns
Pre-packed chromatography columns with unparalleled flexibility in media choice, application, and size
Expert in packing manufacturing-scale columns
>100 45s and 60s shipped since 2015
> cm columns shipped since 2013
1000s of pre-packed columns shipped globally each year (>0.5cm ID)
>100 Phase 1-3 campaigns and 1 commercial process since launch in 2012
>100 different resins packed (8-60cm IDs)
Any resin, any application, any size
Leader in pre-packed columns
Increase process efficiency
Most flexible in resin choice, application, and size (0.5-60cm IDs)
Ready-to-use and disposable—no cleaning validation process
Faster campaign turnarounds
Optimize process economics
Up to 35% cost savings over self- packed columns
Up to 150 FTE hours savings per campaign
No upfront capital expense
Quality and risk mitigation
No column packing failures
ISO 9001 quality manufacturing
Complete documentation package for GMP use

22. XCell™ ATF Systems
Award-winning filtration device that delivers high cell concentration and process intensification
Single-use and stainless steel formats
Leading cell separation device in perfusion mAb and rProtein production
Applications beyond perfusion—process intensification, cell banking, seed train optimization
In 6 of the Top 10 Pharma companies
In 4 commercial and 50+ Phase 1-3 processes
For high density cell culture and process intensification
Finalist 2012 Bioprocess International Technology of the Decade
Increase process efficiency
Consistently high cell retention, concentration, and viability
Easy to use, fewer process steps
Reduced batch size—development scale is commercial-use
Optimize process economics
Reduced capital investment—fewer smaller bioreactors, smaller facilities
Up to 40% reduction in COGs
Quality and risk mitigation
Enables multiple smaller facilities in different regions

23. TangenX™ TFF Cassettes
Proven TFF cassettes in single-use and reusable formats
Reliable, consistent performance since 2007
Used in 200 Phase 1-3 processes and 5 commercial processes
Scalable from bench to production
Interchangeable single-use and reusable formats
TangenX™ SIUS™ and SIUS™ PD Single-use TFF Cassettes
TangenX™ PRO and PRO PD Reusable TFF Cassettes
Two membrane chemistries: ProStream and HyStream
Reliable, scalable, cost-effective solutions for tangential flow filtration (TFF)
First single-use TFF cassettes
Increase process efficiency
Pre-sanitized, ready-to-use
Scalable from bench to production
Faster campaign turnarounds
Optimize process economics
80% reduction in filter costs
75% reduction in buffer use
50% reduction in labor use
Quality and risk mitigation
Complete documentation package for GMP use
Compliant with USP Class VI
BSE / TSE Free

24. CaptivA® Protein A Resin
Most cost-effective Protein A affinity resin available today
Industry-standard results at a fraction of the cost of traditional resins
Secure global supply
In 3 commercial processes, expanding pipeline of Phase 1-3 processes
The value choice in Protein A resins
Protein A resins from the leading manufacturer of Protein A ligands
Increase process efficiency
High dynamic binding capacity, low Protein A leaching
Fastest Protein A quantitation—reliable, robust, reproducible
Optimize process economics
Up to 50% lower than the price of leading resins
Increased savings when pre-packed into OPUS® columns
Quality and risk mitigation
Fully documented, certified ISO 9001 quality system
Animal origin-free, manufactured to USP purity standards
Secure supply chain

25. LONG®R³ IGF-I cell culture growth supplement
Potent and safe cell growth supplement for serum-free or low-serum cell culture
Recombinant, animal-free, cGMP compliant
Used extensively in mAb and rProtein production
Secure global supply
In 6 commercial processes
Increasing adoption in biosimilar development, cell therapy, and vaccine applications
Related products: LONG®EGF growth factor and LONG®R³ IGF-I ELISA Quantitation Kit
Potent, safe, flexible cell culture growth factor
Up to 200 times more potent than insulin
Increase process efficiency
Increased cell density
Increased viability
Increased cell-specific productivity
Optimize process economics
Reduced frequency of growth factor feeds
Improved performance of chemically-defined protein-free media
Reduced processing time
Quality and risk mitigation
Recombinant, animal-free, cGMP compliant
Global safety stock program
Secure supply chain