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Value of the sperm deoxyribonucleic acid fragmentation level, as measured by the sperm chromatin dispersion test, in the outcome of in vitro fertilization.

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Presentation on theme: "Value of the sperm deoxyribonucleic acid fragmentation level, as measured by the sperm chromatin dispersion test, in the outcome of in vitro fertilization."— Presentation transcript:

1 Value of the sperm deoxyribonucleic acid fragmentation level, as measured by the sperm chromatin dispersion test, in the outcome of in vitro fertilization and intracytoplasmic sperm injection  Lourdes Muriel, Ph.D., Nicolás Garrido, Ph.D., José Luis Fernández, M.D., José Remohí, M.D., Antonio Pellicer, M.D., Maria José de los Santos, Ph.D., Marcos Meseguer, Ph.D.  Fertility and Sterility  Volume 85, Issue 2, Pages (February 2006) DOI: /j.fertnstert Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

2 FIGURE 1 Pronuclear test according to nucleolar precursor bodies number and distribution. Description is included in each category. Muriel. Sperm DNA fragmentation and IVF/ICSI outcome. Fertil Steril 2006. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

3 FIGURE 2 Linear regression analysis of the relationship between DNA fragmentation (x-axis) in capacitated sperm cells and fertilization rate (y-axis). Panel represents a scattergram and the regression line. Muriel. Sperm DNA fragmentation and IVF/ICSI outcome. Fertil Steril 2006. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

4 FIGURE 3 Graphic representation of the relationship between fresh and capacitated DNA fragmentation and zygote patterns. (A) Correlation of the number of pronucleus of each zygote with DNA fragmentation (PN = pronucleus). (B) DNA fragmentation is represented according with the PN patterns. (C) Regarding pronuclei size, we compare DNA fragmentation in those of equal or very similar size and those of different sizes. Asterisks (*) denote a significant increase of DNA fragmentation on the type IV pattern compared with the other three patterns (P<.05). Muriel. Sperm DNA fragmentation and IVF/ICSI outcome. Fertil Steril 2006. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

5 FIGURE 3 Graphic representation of the relationship between fresh and capacitated DNA fragmentation and zygote patterns. (A) Correlation of the number of pronucleus of each zygote with DNA fragmentation (PN = pronucleus). (B) DNA fragmentation is represented according with the PN patterns. (C) Regarding pronuclei size, we compare DNA fragmentation in those of equal or very similar size and those of different sizes. Asterisks (*) denote a significant increase of DNA fragmentation on the type IV pattern compared with the other three patterns (P<.05). Muriel. Sperm DNA fragmentation and IVF/ICSI outcome. Fertil Steril 2006. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

6 FIGURE 4 Graphic representation of the scoring of the embryo on day 5 and day 6 and the association with DNA fragmentation. Degree of development on day 5 (A) and day 6 (B). Asterisks (*) denote a significant decrease of fresh DNA fragmentation on the expanded blastocyst pattern compared with blocked or morula stage embryos (P<.05) as well as a decrease in capacitated DNA fragmentation comparing a blastocyst, with its initial cavity, with an expanded blastocyst. CM = compact morula; EaB = early blastocyst; CB = blastocyst with initial cavity; EB = expanded blastocyst; HB = hatching blastocyst. Muriel. Sperm DNA fragmentation and IVF/ICSI outcome. Fertil Steril 2006. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

7 FIGURE 5 Good quality blastocysts were classified as those where the trophoectoderm and inner cell mass (ICM) were complete and with compact and high enough cells, respectively. A comparison of DNA fragmentation of these blastocysts with the rest of the cohort on day 6 was performed. Muriel. Sperm DNA fragmentation and IVF/ICSI outcome. Fertil Steril 2006. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

8 FIGURE 6 Graphic representation of DNA fragmentation in raw and capacitated sperm samples used in the cycles. Samples were divided into two groups: pregnancy or not in the proper cycle. Data were combined and expressed as the mean ± SEM. No statistical differences were found between both groups as compared using the student’s t-test. Muriel. Sperm DNA fragmentation and IVF/ICSI outcome. Fertil Steril 2006. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions


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