1. Test 1: Paper Chromatography

2. Directions:
Use the following images to guide you through the steps of paper chromatography

3. Step 2
With a PENCIL, draw a straight, vertical line that is 2 cm from the BOTTOM of the chromatography paper.

4. Step 3
With a PENCIL, label the TOP part of the chromatography paper “Bc “ (Botana curus), X, Y and Z as shown in Figure 2.

5. Step 4
Using a CLEAN dropper, apply to 2 drops of the Botana curus plant extract just above the penciled line as shown in Figure 2.

6. Step 5
Repeat STEP 4, applying 2 drops of plant extract from the 3 species (X, Y and Z) in the appropriate locations on the chromatography paper.

7. Step 6
Add enough water to cover the bottom of the cup approximately 1 cm deep. See Figure 3 shown below.