1. Supplementary Figure 1 a b c d e
BAY / CHL-1
120
100 80 60 40 20
BAY
absorbance
RLU (%) nM
BAY / SK-MEL-28
120
100 80 60 40 20
Compound log M] Compound log M] Compound log M]
absorbance
JQ1 c nM
BRD4
RLU (%)
Compound log M] Compound log M] Compound log M]
CHL COLO SK-MEL-2 d e
MYC SOX MITF
mRNA expression
level (RMA, log2)
mRNA expression
level (RMA, log2)
mRNA expression
level (RMA, log2)
Representative proliferation curves and gene expression profiles of melanoma models.
(a) Cell proliferation activity of BAY and JQ1 in CHL-1, COLO-792 and SK-MEL-2 cell lines. (b) Colony formation assay of BAY in CHL-1 and SK-MEL-28 cell lines. (c) Basal BRD4 expression in melanoma cells relative to the G-361 cell line level. (d) Comparison of proliferative and invasive transcriptional signatures of melanoma cell lines. Boxes mark cell lines with significantly higher invasive signature. (e) Comparison of MYC, SOX10 and MITF gene expression between sensitive (n = 15) and insensitive cells (n = 5). Data are from GSE Whiskers denote minimum to maximum.