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Detection of imipenem-resistant and metronidazole-resistant Bacteroides fragilis group strains in fecal samples  Hong Fang, Charlotta Edlund, Guangming.

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Presentation on theme: "Detection of imipenem-resistant and metronidazole-resistant Bacteroides fragilis group strains in fecal samples  Hong Fang, Charlotta Edlund, Guangming."— Presentation transcript:

1 Detection of imipenem-resistant and metronidazole-resistant Bacteroides fragilis group strains in fecal samples  Hong Fang, Charlotta Edlund, Guangming Zhang, Maria Hedberg  Clinical Microbiology and Infection  Volume 5, Issue 12, Pages (December 1999) DOI: /j tb00709.x Copyright © 1999 European Society of Clinical Infectious Diseases Terms and Conditions

2 Figure 1 Agarose gel electrophoresis of PCR products from sample 603 for detection of ccrA. Lane 1: purePCR with 1 µL of DNA. Lanes 2–5: imsPCR with 10 µL, 5 µL, 1 µL and 0.1 µL of DNA, respectively. Lanes 6–8: dirPCR with 15 µL, 5 µL and 1 µL of DNA, respectively. Lane 9: DNA molecular size marker (D-15 DNA marker; NOVEX, San Diego, Ca, USA). Clinical Microbiology and Infection 1999 5, DOI: ( /j tb00709.x) Copyright © 1999 European Society of Clinical Infectious Diseases Terms and Conditions

3 Figure 2 The dirPCR products of ccrA from spiked fecal samples prepared with serial dilutions of B. fragilis KSB 1468/90. Lane 1: negative control. Lane 2: positive control. Lanes 3–11: dilutions from 10 to 109 CFU/g of feces, respectively. Lane 12: DNA molecular size marker. Clinical Microbiology and Infection 1999 5, DOI: ( /j tb00709.x) Copyright © 1999 European Society of Clinical Infectious Diseases Terms and Conditions

4 Figure 3 The imsPCR products of ccrA from spiked fecal samples prepared with serial dilutions of B. fragilis KSB 1468/90. Lane 1: negative control. Lane 2: positive control. Lanes 3–11: dilutions from 10 to 109 CFU/g of feces, respectively. Lane 12: DNA molecular size marker. Clinical Microbiology and Infection 1999 5, DOI: ( /j tb00709.x) Copyright © 1999 European Society of Clinical Infectious Diseases Terms and Conditions

5 Figure 4 Agarose gel electrophoresis of PCR products for detection of nim. Lanes 1 and 13: DNA molecular size marker. Lane 2: negative control. Lane 3: positive control. Lane 4: sample 1619 by imsPCR. Lanes 5–8: B. fragilis 1619, B. vulgatus 2985, B. uniformis 2935 and B. thetaiotaomicron 2877 by purePCR. Lanes 9–12: samples 1619, 2985, 2935 and 2877 by dirPCR. Clinical Microbiology and Infection 1999 5, DOI: ( /j tb00709.x) Copyright © 1999 European Society of Clinical Infectious Diseases Terms and Conditions


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